FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_68.351000000427d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_68.351000000427d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249746
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC33111.3257469589102528No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT6120.2450489697532693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACA650.044.999615
TAATTCG207.022979E-444.99964
TGCCGTC4100.043.3532745
CTAGACT301.1374242E-437.499674
TAGACTG301.1374242E-437.499675
TGTGTCG250.002104049535.9996831
ATGCCGT4950.035.9087744
TATGCCG5150.034.51425643
ACACACG850.034.4114612
CCACGAG5250.034.2854120
CGTATGC5250.033.85684241
GAGACTC5250.033.85684224
CCCACGA5350.033.64455819
ATCTCGT5300.033.53743737
GCCCACG5400.033.3330418
GTATCTC5350.033.22400335
ACGACAA950.033.157616
CACGAGA5450.033.0272321
CTAACGT754.7293724E-1132.99970629
CGAGACT5400.032.91637423
Produced by FastQC (version 0.11.2)