FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_66.35100000042792.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_66.35100000042792.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236224
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC100844.26882958547819No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT16900.7154226496884313No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC11000.4656597128149553No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5910.250186263885126No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5520.23367651043077758No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG5140.21759008398807908No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC5100.21589677594147927No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC4950.2095468707667299No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4700.1989636954754809No Hit
GATATATACAACTAGACATTTGTTGGGTTTTATTTAACTGTGTTGCTTTTG4420.18711053914928205No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG4170.17652736385803305No Hit
TGCTATAGCAGTGTTATTGAACAAAAGCAACACAGTTAAATAAAACCCAAC3080.13038471958818748No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC2880.1219181793551883No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA2830.11980154429693848No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA2690.11387496613383906No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC2650.11218165808723923No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC2640.11175833107558927No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA2410.10202180980764021No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGCA207.022059E-444.99978610
TGCCGTC9200.043.53240245
TCACTGG250.00210377735.99983220
GGGTTAC250.00210377735.99983228
CACGTTA250.00210377735.99983220
GAGGGGA250.00210377735.99983215
ATGCCGT11150.035.91911344
CGTATGC11800.034.13119541
TATGCCG11800.034.13119543
GAGACTC11750.033.8934624
GTATGCC11900.033.8443842
GACTCAA11800.033.7498426
ATCTCGT11950.033.7027737
CTCGTAT11950.033.7027739
CACGAGA11950.033.7027721
TATCTCG11900.033.65530436
CGAGACT11850.033.60743723
ACGAGAC12050.033.4230822
GTATCTC12000.033.37484435
CTCAACT11950.033.32620228
Produced by FastQC (version 0.11.2)