FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_64.3510000004275f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_64.3510000004275f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143698
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC60374.201171902183747No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT45363.1566201338919124No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG4880.3396011078790241No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1870.13013403109298666No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1700.1183036646299879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7100.044.04929445
CCCATCT250.002100989736.014
TAGCAGT300.00513245230.0000026
AGCTGGT300.00513245230.00000218
ATGCCGT11500.027.044
ATCTCGT12350.025.32388737
CTCGTAT12200.025.26639439
TATGCCG12200.025.26639443
TCTCGTA12300.025.24390238
GAGACCT12750.025.23529424
AAATCTC12500.025.19999935
CGAGACC12500.025.19999923
GACCTGA12600.025.17857226
AATCTCG12550.025.09960236
TTAAATC12650.025.0790533
CGTATGC12300.025.06097641
GTATGCC12300.025.06097642
ACCTGAT12750.025.05882527
CCTGATT12750.025.05882528
TTGCATG450.001221335425.0000023
Produced by FastQC (version 0.11.2)