FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_63.35100000042735.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_63.35100000042735.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219141
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC146516.6856498783888No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT48922.2323526861700915No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG7050.3217106794255753No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4950.22588196640519115No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4030.18389986355816576No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2930.1337038710236788No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2490.11362547400988404No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2460.11225649239530713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15900.044.0063145
ATGCCGT21300.032.74415644
TATGCCG22600.030.86063843
CTCGTAT22350.030.70251739
ATCTCGT22300.030.6704737
GTATGCC22600.030.66153742
CCTGATT22700.030.62558228
TCTCGTA22450.030.5657638
CGTATGC22750.030.55827141
CTGATTA22750.030.55827129
GAGACCT22900.030.554624
CACGAGA22850.030.52299721
CGAGACC22850.030.52299723
AATCTCG22450.030.46554436
GACCTGA22900.030.45635426
AAATCTC22550.030.43021235
ACGAGAC22950.030.39000122
CCACGAG22950.030.39000120
TCGTATG22750.030.36048340
ACCTGAT22900.030.35810727
Produced by FastQC (version 0.11.2)