FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_62.3510000004271b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_62.3510000004271b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326606
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC40661.2449250779226346No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT16940.5186677525826225No Hit
GTATACACACAACACACGACAAACTATTCTAACGTATTCACACATTGACAG3820.11696049674531392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4550.042.52573845
GAGCCAA356.2363088E-638.5698615
GAGTCCA301.1384071E-437.498479
CGGTCGT250.002105327335.9985310
GGTCGAG352.8166609E-432.1415511
TACTCGC352.8166609E-432.1415526
CGAGCCA352.8166609E-432.1415514
TGGTCGA352.8166609E-432.1415510
CGTACTG300.005139105530.0033685
AGTGCTC300.00514295529.99877510
TCGGTCG300.00514295529.9987759
ATGCCGT6650.029.09655644
CGTATGC7050.027.44568841
TATGCCG7050.027.44568843
CCACGAG7050.027.44568820
GTATGCC7100.027.25240742
CGAGACC7150.027.0618323
GAGACCT7000.026.998924
GGTTGTC1500.026.9988987
ATCTCGT7200.026.87390337
Produced by FastQC (version 0.11.2)