FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_61.351000000426f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_61.351000000426f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63434
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC20703.2632342277012323No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT1940.30582968124349713No Hit
CCATTAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG1730.2727244064697165No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG1390.21912539016930985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.2143960652016269No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1300.204937415266261No Hit
GCCTCTAACCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTG1250.1970552069867894No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.18917299870731785No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.174985023804269No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.15449128227764292No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCTCA950.14976195730995998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.145032632342277No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGTCGTTAAGTGTCTTGCCCA890.14030330737459407No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT890.14030330737459407No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCCCA800.12611533247154524No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA780.1229624491597566No Hit
ACCTCTGGGTTACAGGCAGGCGCGCTAACCACTACGCCACCTGTCTCTTAT770.12138600750386229No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA760.11980956584796795No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC750.11823312419207364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.11508024088028501No Hit
GTGGCGTAGTGGTTAGCGCGCCTGCCTGTAACCCAGAGGTCTGTCTCTTAT680.10719803260081345No Hit
CCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG640.10089226597723618No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAT206.986534E-444.9952744
TGCCGTC2150.040.8096645
ATGCCGT2550.034.40814644
AATCTCG2600.033.74645236
ATCTCGT2600.033.74645237
TAAATCT2600.033.74645234
CTCGTAT2600.033.74645239
CCCACGA2700.033.3298319
GTATGCC2650.033.10972642
AAATCTC2650.033.10972635
TATGCCG2650.033.10972643
TGATTAA2650.033.10972630
CCTGATT2650.033.10972628
CTGATTA2650.033.10972629
TTAAATC2650.033.10972633
CCACGAG2800.032.94296620
CACGAGA2750.032.7238321
GAGACCT2750.032.7238324
TCGTATG2700.032.49658240
GATTAAA2700.032.49658231
Produced by FastQC (version 0.11.2)