FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_60.351000000426d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_60.351000000426d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244133
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC6582226.961533262606856No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT123315.0509353508128765No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG51932.127119234187922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATG207.010082E-445.0159843
TGCCGTC70200.044.70909545
ATGCCGT83450.037.5833344
CTTCCGA250.002100200236.0127871
CCGATGT250.002100200236.0127874
TTGTCAC250.002104429535.99803514
CCGTACA250.002104429535.99803517
CGTATGC89250.035.14093841
GTATGCC89200.035.13541442
TATGCCG89250.035.11572643
ATCTCGT89350.035.1016137
TCTCGTA89350.035.1016138
CTCGTAT89400.035.08197439
TCGTATG89350.035.02606640
AAATCTC89700.034.98972735
GACCTGA89700.034.9144826
AATCTCG89900.034.9118936
CTGATTA89450.034.9114529
TGATTAA89600.034.87811730
CGAGACC90000.034.87309323
Produced by FastQC (version 0.11.2)