FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_6.35100000042005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_6.35100000042005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27163
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC646723.808121341530757No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT6482.385598056179362No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG1380.5080440304826418No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG450.16566653167912232No Hit
GAATAAAATACTTAGGAACAAATTTAATGAAAGCAGCGCATACAAGACTTG400.14725913927033096No Hit
GATAAACATATCTTTCGAGGAGTTTTATCTAGTATACGTACATTCTTCTCT380.13989618230681442No Hit
ACACATTTTTTATTCAAAGTTTTCCAACATCGGTAATCTCGAAAAATCAAA360.13253322534329787No Hit
ACATTCATTATAATCTGGCTGAATTAATTGCTGAAGCAAAGGATAAAAATA310.1141258329345065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7200.044.062545
ATGCCGT7800.040.67307744
AGCCCAC8050.039.96894517
ATGCGTT8050.039.96894530
GAGCCCA8050.039.96894516
CTCCGAG8050.039.96894512
CAATGCG8000.039.937528
CCCACGA8000.039.937519
ACGAGAC8000.039.937522
TCTCCGA8000.039.937511
AATGCGT8000.039.937529
ACAATGC8000.039.937527
CGAGACA7950.039.90566323
GTTATCT7950.039.90566334
GCCCACG8100.039.7222218
CGAGCCC8100.039.7222215
CCGAGCC8100.039.7222214
CGTTATC8050.039.6894433
ATCTCCG8050.039.6894410
CACATCT8050.039.689447