FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_59.351000000426bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_59.351000000426bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172317
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC85824.9803559718425925No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT13690.7944660132198217No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5860.34007091581213694No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4560.26462856247497346No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.22806803739619425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.22690738580639172No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3320.19266816390721753No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.19266816390721753No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG3180.1845436027785999No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.1764190416499823No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2860.16597317734175968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.15494698723863579No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2580.14972405508452447No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.1421798197508081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.13985851657120307No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2010.11664548477515277No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1900.11026190103123894No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.10213733990262132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8200.043.90244345
ATGCCGT9800.036.73469544
GGTGTGG250.00210215235.9999966
CTGTTGT250.00210215235.99999627
ATTGCGA250.00210215235.99999611
CGTATGC10350.034.78260841
CTCGTAT10400.034.61538339
TATGCCG10400.034.61538343
GTATGCC10400.034.61538342
TCTCGTA10450.034.4497638
CGAGACC10400.034.39903623
ACGAGAC10450.034.2344522
GAGACCT10550.034.12322624
AATCTCG10550.034.12322636
GACCTGA10550.034.12322626
CACGAGA10500.034.0714321
CCACGAG10500.034.0714320
CTGATTA10500.034.0714329
AGACCTG10700.033.6448625
TCGTATG10700.033.6448640
Produced by FastQC (version 0.11.2)