H23LNBCXX_l01n01_bir_1228_pb_58.35100000042693.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pb_58.35100000042693.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	212115
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	11121	5.242910685241496	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	1620	0.7637366522876742	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT	1341	0.6322042288381302	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC	862	0.4063833297975155	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	834	0.39318294321476555	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	831	0.3917686160808996	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC	684	0.32246658652146243	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT	673	0.3172807203639535	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG	665	0.31350918134031064	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC	407	0.19187704782782924	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	405	0.19093416307191854	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC	395	0.186219739292365	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	381	0.17961954600099003	No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA	376	0.17726233411121325	No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC	354	0.16689060179619547	No Hit
ACATACAACAATGAATGAAGTTTCAAGTGATGAAGAATTGGATCCATCAAC	354	0.16689060179619547	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCG	342	0.16123329326073121	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA	342	0.16123329326073121	No Hit
TTATAACATACAACAATGAATGAAGTTTCAAGTGATGAAGAATTGGATCCA	333	0.156990311859133	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA	321	0.15133300332366878	No Hit
ATTTATAACATACAACAATGAATGAAGTTTCAAGTGATGAAGAATTGGATC	308	0.14520425241024917	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	304	0.14331848289842775	No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG	297	0.14001838625274024	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	293	0.13813261674091884	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT	286	0.13483252009523136	No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT	284	0.13388963533932063	No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT	279	0.13153242344954388	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	278	0.1310609810715885	No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG	252	0.11880347924474932	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	252	0.11880347924474932	No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG	247	0.11644626735497253	No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC	243	0.11456049784315113	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	235	0.11078895881950829	No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA	231	0.10890318930768686	No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC	230	0.10843174692973152	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	224	0.1056030926619994	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCAG	20	7.0202677E-4	45.000004	29
CATATCG	20	7.0202677E-4	45.000004	6
TATGGGT	20	7.0202677E-4	45.000004	30
GCGGGTG	20	7.0202677E-4	45.000004	36
TCCCATG	20	7.0202677E-4	45.000004	26
CTGATAG	20	7.0202677E-4	45.000004	34
ATCGCCA	20	7.0202677E-4	45.000004	18
TCGAAAG	20	7.0202677E-4	45.000004	20
ATCTGAC	20	7.0202677E-4	45.000004	3
GAGTATG	25	3.8800194E-5	45.0	15
TCTAGGA	25	3.8800194E-5	45.0	10
ACGATCG	45	3.8198777E-10	45.0	44
GCTAGCA	25	3.8800194E-5	45.0	44
TACTCGA	25	3.8800194E-5	45.0	36
CTCGAGA	25	3.8800194E-5	45.0	38
TGCTAGC	25	3.8800194E-5	45.0	43
TCGAGAT	25	3.8800194E-5	45.0	39
GTCCACT	25	3.8800194E-5	45.0	11
CGATCGG	45	3.8198777E-10	45.0	45
CTAGGAG	25	3.8800194E-5	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content