FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_56.35100000042650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_56.35100000042650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202657
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC20821.0273516335483106No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT7740.38192611160729706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG4730.23339929042668156No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA2990.14753993200333568No Hit
AAGGAATGTGACAATAACGCAAGAAACCTGTCTCTTATACACATCTCCGAG2630.1297759268122986No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA2410.1189201458622204No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC2390.11793325668494056No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTGTCTCTTATACA2170.10707747573486236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2000.043.87187245
CCTGAGT250.0021037635.9974338
ATGCCGT2650.033.11084744
ACTATTC352.8134152E-432.14056423
TGAGGTT352.8134152E-432.1405645
TATGCCG2900.030.25646443
TCCTGAG300.005139167429.997867
TATCTCG3000.029.24791536
CGAGACG3000.029.24791523
CCACGAG3000.029.24791520
ACGATCG3050.028.77554127
ACGAGAC3050.028.7684422
GAGACGA3100.028.31141924
CGATCGT3100.028.31141928
GCCCACG3100.028.30443618
GATCGTG3150.027.8620329
CGTGTAT3200.027.42668532
ATCTCGT3200.027.41991837
AGCCCAC3200.027.41991817
CCCACGA3200.027.41991819