FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_55.35100000042636.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_55.35100000042636.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48277
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC3210.6649128984816786No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT2090.4329183669242082No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC1440.2982786834310334No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCCTGTCTCTT1370.28377902520869147No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA1320.27342212647844727No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGCTGTCTCTT1290.26720798724030076No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT1130.23406591130351928No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG1070.2216376328272262No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCC990.20506659485883547No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC950.1967810758746401No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT950.1967810758746401No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATT870.18021003790624934No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC790.1636389999378586No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA750.15535348095366322No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT730.15121072146156556No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA700.14499658222341902No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGG690.14292520247737017No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATAC650.1346396834931748No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG640.13256830374712597No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA620.12842554425502828No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT620.12842554425502828No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC610.12635416450897943No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC570.11806864552478405No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGCTGTCTCTT550.11392588603268637No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG530.10978312654058868No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTC510.105640367048491No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT500.10356898730244217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC206.966232E-445.045
AGGAAAA250.002087207336.034
CCAGAGC352.7807843E-432.1428573
CAGAGCC352.7807843E-432.1428574
TATCCCT602.3387292E-730.00000238
GGAAAAG300.005099099630.00000235
TAGCGCG453.4227494E-530.014
GTATCAA1350.029.9999981
TAGTGGT507.0196795E-527.07
CCTGTAA507.0196795E-527.025
CTGTAAC507.0196795E-527.026
GGTATCA607.981123E-626.2500021
GTATCCC709.0018693E-725.71428537
GTTAGCG551.3418638E-424.54545412
AGCGCGC551.3418638E-424.54545415
GCCTGTA551.3418638E-424.54545424
TTAGCGC551.3418638E-424.54545413
CGATGAG1850.023.10810914
TGAGTAG1850.023.10810917
ATGAGTA1850.023.10810916
Produced by FastQC (version 0.11.2)