FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_54.3510000004260f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_54.3510000004260f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42097
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC23895.67498871653562No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT9502.2566928759769107No Hit
GTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGAAACTACATCTCT3340.793405705869777No Hit
GTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGAAACTACATCT2300.5463572226049362No Hit
GTATGTGTCGGCTCCATCTTCTCATTGAGCAATTGGTGAAGAGATGTAGTT1920.4560895075658598No Hit
GTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGAAACTACAT1660.3943273867496496No Hit
GTATGTGTCGGCTCCATCTTCTCATTGAGCAACTGGTGAAGAGATGTAGTT1220.28980687459914006No Hit
AATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATG1180.2803050098581847No Hit
GGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGACACCTT1160.27555407748770694No Hit
TATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGGA930.22091835522721334No Hit
GAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGACACCTTACTACA900.21379195667149678No Hit
GATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGACACCTTACTACACA730.17340903152243628No Hit
CCTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGAT670.15915623441100316No Hit
GAGCAATTGGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATG650.15440530204052547No Hit
ATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACATGG650.15440530204052547No Hit
CTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATA630.14965436967004775No Hit
ATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGATACA620.1472789034848089No Hit
ATTGCATTGTATGTGTCGGCTCCATCTTCTCATTGAGCAACTGGTGAAGAG570.13540157255861462No Hit
GAGCAACTGGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATG560.13302610637337578No Hit
CTTTAACCCTTAACCTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGA560.13302610637337578No Hit
ATTGCATTGTATGTGTCGGCTCCATCTTCTCATTGAGCAATTGGTGAAGAG550.1306506401881369No Hit
CCCTTAACCTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAAC530.12589970781765922No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT520.12352424163242035No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA480.11402237689146495No Hit
GCAACTGGTGAAGAGATGTAGTTTCCATGTATCGGTAGTTATTTCGATGGA450.10689597833574839No Hit
ACCTATAATATAGGCAGTGTGTAGTAAGGTGTCCATCGAAATAACTACCGA450.10689597833574839No Hit
GCATTGTATGTGTCGGCTCCATCTTCTCATTGAGCAATTGGTGAAGAGATG440.10452051215050953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3300.042.9420645
GTTAATA250.002087131435.9895363
TATGGTA250.002087131435.98953610
CATCTCT401.5282889E-533.7401945
TCTCGTA4400.031.69533238
TATCTCG4400.031.69533236
CGTGTAT4450.031.37651332
ATCTCGT4450.031.33920537
CTCGTAT4450.031.33920539
AGACGAT4550.031.1818725
GACGATC4550.031.1818726
GTATGCC4550.031.1447942
TATGCCG4550.031.1447943
GTGTATC4500.031.02788433
TCGTGTA4500.031.02788431
CGATCGT4500.031.02788428
ATGCCGT4600.030.8062644
CGTATGC4600.030.8062641
GAGACGA4600.030.8062624
GTATCTC4550.030.6869235
Produced by FastQC (version 0.11.2)