FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_53.351000000425e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_53.351000000425e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202736
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG8240.40643990213874204No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC8140.40150737905453393No Hit
GCCTCTAACCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTG5640.27819430194933314No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCCCA4330.21357824954620785No Hit
ACCTCTGGGTTACAGGCAGGCGCGCTAACCACTACGCCACCTGTCTCTTAT4270.21061873569568307No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCTCA4240.20913897877042065No Hit
GTGGCGTAGTGGTTAGCGCGCCTGCCTGTAACCCAGAGGTCTGTCTCTTAT4210.20765922184515823No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT3160.15586772946097388No Hit
CCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG3080.15192171099360746No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC2990.14748244021782023No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGTCGTTAAGTGTCTTGCCCA2910.1435364217504538No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA2850.140576907899929No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT2090.1030897324599479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC659.094947E-1238.07525345
GTACAAC250.00209584636.025081
GATAACA401.554725E-533.7485228
CCTGTCG352.8088936E-432.149383
GGGCGAT300.005119906330.02091
ATTGGTG300.00513228330.0060845
TATAGCT300.005138480629.99868435
AGTATGT554.136191E-628.6421722
AACATTC406.1500736E-428.12376631
GTTCGAG406.1500736E-428.12376615
TCGAGGC406.1500736E-428.12376617
GTGGCGT3750.027.6192281
GGCGTAG4000.027.0054783
TGATAAC507.131969E-526.99881627
CGTGATA507.131969E-526.99881625
CCCGTGA507.131969E-526.99881623
ATAACAT507.131969E-526.99881629
ACCCGCC507.131969E-526.9988168
CCCGCCG507.131969E-526.9988169
TATTGTG856.4010237E-926.4759583
Produced by FastQC (version 0.11.2)