FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_52.351000000425d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_52.351000000425d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210391
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC16810.7989885498904422No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT3960.1882209790342743No Hit
CCATTAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTGGGCGTATG3950.1877456735316625No Hit
CATTAAATCAGTTATGGTTCATTTGATCGTATGGTTTACTTGGATAACTGT3570.16968406443241393No Hit
CTAATGGGTTGTCTAAATTGTAAGCCATATCAAATAAAATCATACACAAAG3340.15875203787234243No Hit
TCTTAATATAGGTCAATATATGAATGCTCTCTTTACAGTCTGTCTCTTATA3200.1520977608357772No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCTCA3130.14877062231749455No Hit
ACTGTAAAGAGAGCATTCATATATTGACCTATATTAAGACTGTCTCTTATA2920.1387892067626467No Hit
GCCTCTAACCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGTG2740.13023370771563422No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTAAGTGTCTTGCCCA2580.12262881967384537No Hit
CTTCTCTATTAAATAGGGTAAAAGTAGTTGAAAACTTAAACGAGGTTAATA2420.1150239316320565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1600.045.00594345
ACACGAC403.4431469E-739.37084214
GTGCTAG250.002096938736.0218851
GCTAGAC250.002096938736.0218853
GAATCGC250.002101841436.00475745
GTCTGGT250.002104296335.9961979
GATCCGC250.002104296335.99619715
CCAGAGT250.002104296335.99619730
GGGGTAT250.002104296335.99619717
GCGATCC250.002104296335.99619713
CTGGGTA250.002104296335.99619736
TAGGTAT352.802584E-432.1623955
ACGGGGG352.802584E-432.1623953
CGAACGC352.81042E-432.14710244
TCTCTAT501.9621257E-631.5191483
CTCTATT501.9621257E-631.5191484
CACGACA501.973087E-631.49667415
CTAACGT501.973087E-631.49667429
CTAGACC300.005122564330.0182344
TAGACCG300.005122564330.0182345
Produced by FastQC (version 0.11.2)