FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_5.35100000041fed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_5.35100000041fed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427246
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC99582.330741540002715No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT13610.3185518413279469No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.12966768559565214No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5410.12662494207084443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG207.029925E-444.99789442
TGCCGTC11350.043.8085245
ATGCCGT13150.037.8119244
GCGTTAT13350.036.91270432
CCACGAG13700.036.78229520
CGTTATC13350.036.74415233
ATCTCGT13450.036.46669437
CAATGCG13650.036.43113328
CGTATGC13650.036.42686541
TATGCCG13650.036.42686543
TGCGTTA13600.036.39961631
ATGCGTT13750.036.32982330
CCCACGA13900.036.25304819
CTCGTAT13650.036.0972139
AATGCGT13800.036.0351429
GTATGCC13850.035.90084542
CACGAGA14000.035.8376121
TCTCGTA13750.035.83468638
GACAATG14000.035.6810826
ACGAGAC14200.035.6539122
Produced by FastQC (version 0.11.2)