FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_48.35100000042544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_48.35100000042544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153711
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC8660.5633949424569484No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT7010.4560506404876684No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG2380.15483602344659786No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG206.983265E-445.040381
TTAGGAC206.994481E-445.0257153
GCCGTCT1200.044.9964216
TTGTGCG207.016957E-444.9964189
CTGCTTG1350.039.9968224
CCGTCTT1350.039.9968217
TAGGACC250.002095558736.0205734
CTTAGGA250.002095558736.0205732
GGACCGT250.00209891136.008856
GGGCTTC250.002102267235.99713537
GACCTCA250.002102267235.99713513
TACCGGG250.002102267235.99713532
GGGGCTT250.002102267235.99713536
GCTTGAA1550.034.83593826
CGTCTTC1600.033.74731418
TGCTTGA1650.032.7246725
GTCGTAT300.0051110830.0269181
AGGACCG300.00512738630.0073765
CCGGGGC300.00513555529.99761434
TACGGCC300.00513555529.99761419
Produced by FastQC (version 0.11.2)