FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_47.3510000004252a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_47.3510000004252a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114630
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC1374211.988135741079997No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG76536.676262758440199No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT38163.3289714734362734No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA2240.19541132338829278Illumina PCR Primer Index 8 (96% over 27bp)
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA2120.1849428596353485No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGC1800.15702695629416383No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACATCTCCGA1460.1273663089941551Illumina PCR Primer Index 8 (96% over 27bp)
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG1340.11689784524121086No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA1320.11515310128238682No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC1300.11340835732356278No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG1230.10730175346767862No Hit
CCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1230.10730175346767862No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTC207.0070394E-444.9995618
GCCGTCC207.0070394E-444.9995616
GTCTTCT20050.044.66290719
GCCGTCT20100.044.55180716
CTTCTGC20000.044.5495721
CGTCTTC20200.044.44263518
CCGTCTT20200.044.44263517
TCTGCTT19900.044.43424223
TGAAAAA19950.044.210129
TTCTGCT19850.044.2061222
TGCTTGA19600.044.19625
CTGCTTG19850.044.09277324
CTTGAAA19700.043.97165327
TTGAAAA19700.043.97165328
TCTTCTG20300.043.891220
GCTTGAA19700.043.8574426
GAAAAAA20150.043.54796230
GCCTGAA501.0641088E-940.49960726
TACCATG250.002099319435.999657
CCGTCCT250.002099319435.9996517
Produced by FastQC (version 0.11.2)