FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_45.351000000424f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_45.351000000424f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT52.8901734104046244No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC52.8901734104046244No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC21.1560693641618496No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG21.1560693641618496No Hit
TTATTGTTCCTCGAGTTCGAACTATCGTGATGATATTTGAACTGTCTCTTA10.5780346820809248No Hit
ATTCAAATATCATCACGATAGTTCGAACTCGAGGAACAATAAGATGTATCA10.5780346820809248No Hit
CAGTAATTCATCATTTCAAGCTTTTCACCGAAGGCTTTCAAGTTCCTGCTG10.5780346820809248No Hit
CTTTGTGCACCTGTTCCCTTCTCATTACGAGCCGAACTTTGGCATTCACTT10.5780346820809248No Hit
CTTTAGATCTGAGTTCTCCGGCTTTGGATACGAACTGTCTCTTATACACAT10.5780346820809248No Hit
GTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCT10.5780346820809248No Hit
CATCACGATAGTTCGAACTCGAGGAACAAAGATGTATCATCAGAAGTTTCT10.5780346820809248No Hit
TGTTTCATGTTTTACTGTCACCGTTTCTCCTTTTTGCTTACTCTTCTCGAA10.5780346820809248No Hit
CCGTGAAGTTGCTACATCCAACAAGGGACAGGGTCTTAGCTTCCTTCTAGG10.5780346820809248No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATACGCCTCGC10.5780346820809248RNA PCR Primer, Index 36 (96% over 29bp)
TCTTATACACATCTCCGAGCCAACGAGACAGATATAAATCTCGTATTCCGG10.5780346820809248No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT10.5780346820809248No Hit
CACATGGCTCATTTGAGATCCTCCGCCAGCGCGTCTTCCAGCTCTCTTAAC10.5780346820809248No Hit
CTTCGAAATAATGGATTTACTTCAGTGAGGTGTATGAGCTGTCTCTTATAC10.5780346820809248No Hit
AATTTGGACAACCCATTAGTGACCACTGGGTTGGAGCTGTCTCTTATACAC10.5780346820809248No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCACTTATACAC10.5780346820809248No Hit
ACTCTGCGTTGATACCACTGCCCCGTACTCTGCGTTGATACCCTGTCTCTT10.5780346820809248No Hit
CCGTCAAGTGTCTTGCTCAAGGACACATACGCCCACAATGGTAGCAGCGAC10.5780346820809248No Hit
TTTTTTTTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTC10.5780346820809248No Hit
GCATAACATGACAAAAAGCACAAAGGCTCATTCCTGTCTCTTATACACATC10.5780346820809248No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAG10.5780346820809248No Hit
GAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACTGTCT10.5780346820809248No Hit
CATTATTACTCAGATAGCGTACGCCCGAATTGAGGGAGATGTTGTCATATG10.5780346820809248No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTAAAAAAAACTGTCTCTTATAC10.5780346820809248No Hit
GTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTT10.5780346820809248No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCTACGCAGAGTACGGGAAGCA10.5780346820809248No Hit
TTTTTTTCTGCGTTGATACCCCCGTACTCTGCGTTGATACTGTCTCTTATA10.5780346820809248No Hit
TTTCCAGTCTTCATGCGCACCCATTGTGGGATGGGCTGTCTCTTATACACA10.5780346820809248No Hit
TCTTAAAGCAACTCAAAGCTCAAATCGAAATTTCTAGTTTCTCTTTATCAT10.5780346820809248No Hit
TGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACAACCCCG10.5780346820809248RNA PCR Primer, Index 31 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGT10.5780346820809248No Hit
GACGCAAAGATGAGCCTGGCGTTTATCCAAGGCCCTTGCACATTCGTTCAA10.5780346820809248No Hit
CTCTTCACCTTAGAACCCTTCTTGAAGACAAAAGATTTGGAAAGTGTCGTA10.5780346820809248No Hit
GACCAAGGTTAATGAACAACAACAATGTTGCTCATCCAGAAACCTGTCTCT10.5780346820809248No Hit
CACTAAGAAAGTTGGTTCCATCCCAAAATGTGGTGACACTAAGAGACAATT10.5780346820809248No Hit
ATACCACTGCTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC10.5780346820809248No Hit
TCATTGCAAAATCTTGTTGCTGATACAAATCAGTACAAAGCAACATGAACG10.5780346820809248No Hit
TTATTATAGCAGATATACAGCGCTTTCAAAATTTAAAATGCATAAAGCATA10.5780346820809248No Hit
GAATCATTTGTCCCTTTCTCTTTCTATCACCTCCCAACTCGAAGTGCTTAC10.5780346820809248No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACGACTGCCG10.5780346820809248Illumina PCR Primer Index 5 (96% over 28bp)
CTATGAAGAGAGAGTAGACCTTCTTAATGTCTTCCACCTGTCTCTTATACA10.5780346820809248No Hit
ACCTTAAATCAACGGTGGAGGATGACAAAGTGAAAGATAAAATTTCAGACG10.5780346820809248No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACGCCAAACA10.5780346820809248Illumina PCR Primer Index 8 (96% over 27bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATACACATCTC10.5780346820809248RNA PCR Primer, Index 27 (96% over 28bp)
ATTAAAAACACAGCACTGTGCAAACACGAAAGTGGACGTATACCTGTCTCT10.5780346820809248No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCTGTCTCTTATACACATCTCC10.5780346820809248No Hit
TTCTCAAAGCAAAGGGACTTGCCCCAGATCTTCCTGAAGATATTTACCATC10.5780346820809248No Hit
AAAAAAAGTACTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGT10.5780346820809248No Hit
AAGGAATGTGACAATAACGCAAGAAACCTGTCTCTTATACACATCTCCGAG10.5780346820809248No Hit
GAGTACGGGGTGGTATCAAGCAGTGGTAAAAAACTGTCTCTTATACACATC10.5780346820809248No Hit
ATCCATACACTGAATCTTCTTTCTGTTGTGTTACCGCTTGCCAACACTTTA10.5780346820809248No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACATACATCCTC10.5780346820809248RNA PCR Primer, Index 36 (96% over 29bp)
GGCCAACACAATAGGATCGAAATCCTATGATGTTATCCCTGTCTCTTATAC10.5780346820809248No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACTTCATCGCCTC10.5780346820809248Illumina PCR Primer Index 5 (96% over 28bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAA10.5780346820809248Illumina PCR Primer Index 8 (96% over 27bp)
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCTGTCTCTTATACAC10.5780346820809248No Hit
CTCCAAAACTGGTAATTTTGGATTTGGAATCCAGGAACACATTGATTTGGG10.5780346820809248No Hit
AAAGTAAAAAGTACTCTGCGTTTACCCCCGTACTCTGCGTTGATACTGTCT10.5780346820809248No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAACTGTCTCTTATACACA10.5780346820809248No Hit
GGTAACCAGACCACCAACTCGATAGGGTCAACATCGTGTGCAATGACCACA10.5780346820809248No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTCCGAGCCCA10.5780346820809248RNA PCR Primer, Index 27 (96% over 28bp)
CAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCTCCG10.5780346820809248No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATATTCC10.5780346820809248No Hit
CTATTTCAATACACAGTTATGCTTTTGCCTGTGATTTACTTTGGCGTGGTT10.5780346820809248No Hit
CTGCAAACACTTCTTAACCCAAGCAATCAGTTTCGGAACCTCAGATTCGAT10.5780346820809248No Hit
TTTTTTTTTGCGTTGATACCACACCCGTACTCTGCGTTGATACTGTCTCTT10.5780346820809248No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCCTGTCTCTTATACACATCTCC10.5780346820809248No Hit
TCGCAATCCCATTTGTATTGACGCGAATACTAAAAGGAAACTCCCATTTAC10.5780346820809248No Hit
GTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCCGAGCC10.5780346820809248No Hit
ATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCT10.5780346820809248No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT10.5780346820809248No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT10.5780346820809248No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA10.5780346820809248No Hit
GCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTT10.5780346820809248No Hit
GTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTC10.5780346820809248No Hit
GTGAGCTTCAGCGAAGTCGTCCAAATCATCAACCATAAACCTGGGTAAAGA10.5780346820809248No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA10.5780346820809248No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT10.5780346820809248No Hit
ATCTAAAGGAATGACGTCAGAGTTTCCTTCTTAAGCAAAGAAAGGATAGAC10.5780346820809248No Hit
CTTGGGAATAATACATCGTCTACTTCATCAAGTCTAATCACTGTCTCTTAT10.5780346820809248No Hit
TTTTTTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCCGTACTCCT10.5780346820809248No Hit
CTTGTCCTCGGACTTCTTGTTCTCTCTGAAACCACGAATGCGTTCCTGTCC10.5780346820809248No Hit
GAACAGCAACAACTTTAAAAATCATGTTTTGATTATACGAATACCTCCTAC10.5780346820809248No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTA10.5780346820809248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTT10.5780346820809248No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAATAATACCATT10.5780346820809248RNA PCR Primer, Index 27 (96% over 28bp)
CTTTTTTAGCCTTTGTTACTTTCACTGGTTTTATCACCTGTCTCTTATACA10.5780346820809248No Hit
ATATGAGGGAGTGGTTCAGAGGCATAGTGCAAGCACTGTCTCTTATACACA10.5780346820809248No Hit
GTATGTCATAACCGGCAGGATTACCCTGTTCATTGTACTCATATACTGTCT10.5780346820809248No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGCCATCAC10.5780346820809248Illumina PCR Primer Index 8 (96% over 27bp)
CCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCCTGTCTCTTATACA10.5780346820809248No Hit
CAGGAATACTGAGTGACAAAGCTCAAGAACTGTCTCTTATACACATCTCCG10.5780346820809248No Hit
GTTCATGTTGTAGTCAATACAAGGGTACGTATTGTCCCTGTCTCTTATACA10.5780346820809248No Hit
GCTCAAGGCGTAAGGTGCTGCGGTGCAGCGAAGTCCCATTTCTCAAAATCA10.5780346820809248No Hit
GTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACCAAACAACACAAACG10.5780346820809248Illumina Single End Adapter 1 (95% over 22bp)
TAACACTTCTCAGGATTCAGGGATTCATGCTAGACATATAAGTACCTGTCT10.5780346820809248No Hit
ACATAGTGTTAAAACATAAGATCACTCTGGCAGCTCCTGTCTCTTATACAC10.5780346820809248No Hit
GTTGTTATTACTGGAACAAAACAAAATAGCGAGACATTTAGACCTTCTTAA10.5780346820809248No Hit
CTTATATTGTGACGTCATTTGTTATCCACTTTGACAACTGTCTCTTATACA10.5780346820809248No Hit
AACGAAAATTGCTCCAACCCAGTGGTCACTAATGGGTCTGTCTCTTATACA10.5780346820809248No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC10.5780346820809248No Hit
TTTTTGTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGT10.5780346820809248No Hit
TTACCACAGTTATCCAAGTAAACCATACGATCAAATGAACCATAACCTGTC10.5780346820809248No Hit
GAATCAGCAGGTCGTAGGCCTTGCGGTCGGCATAGGGGCCATCGTCGCCGT10.5780346820809248No Hit
TTTTATGATACCACTGCTTCCCGTACTCCTGTCTCTTATACACATCTCCGA10.5780346820809248No Hit
GTAGACATCATCGGACTTAGGAGCAGTCCTTTTGGTCTTCTTACTCTTACC10.5780346820809248No Hit
ACGTAGATGATGATAAACTTAACATGGTCATCAAGGAGTTGAAGGGGAAGA10.5780346820809248No Hit
TATCAACGCAGAGTACGGGAAGCAGTGATATCCTGTCTCTTATACACATCT10.5780346820809248No Hit
ACTCTTGACTAGTCTTGAAAGCTTCTTGTGATTTCCTGTCTACAATCTGTC10.5780346820809248No Hit
TATCAACGCAGAGTACGGGACGCAGAGTACTTTCTGTCTCTTATACACATC10.5780346820809248No Hit
TTATTACGCTGAGTTGGATGCAGTTGAACTCACAGGCACGCTTGAGTTCAG10.5780346820809248No Hit
GGGTAAAAGGCCAACCAAGATCGGATATAGCTGGTTTTCCGCGAAATCTAT10.5780346820809248No Hit
GTATGTAGGAACTTCCAAGTCCATACAAATGGCCACAAAATTTCGGAATAT10.5780346820809248No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTC10.5780346820809248No Hit
AAAAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA10.5780346820809248No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTG10.5780346820809248No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT10.5780346820809248No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA10.5780346820809248No Hit
TTTCATCGGTTAACTCTGTCGTATAACTCTCAAATTCTTCACTGTCTCTTA10.5780346820809248No Hit
GAATACGATCGTAACGAAGAAGTATATCTTTGCCATCATCATTTTTGATGA10.5780346820809248No Hit
ACCCATTAGTGACCACTGGGTTGGAGCAATTGCCGTTCTGTCTCTTATACA10.5780346820809248No Hit
GAGAAGAATCATGAGATGATCACCGGTTTGAAGAACGCGATGAAGTTGAGA10.5780346820809248No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTATTTTCTGTCTCTTAT10.5780346820809248No Hit
ATATAAATCTCGTATGCCGTCTTCTGCCTGAAAAAAAAAAAAAATACCGTC10.5780346820809248Illumina PCR Primer Index 4 (96% over 25bp)
GTATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTG10.5780346820809248No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAT10.5780346820809248No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACAAAAATCTCGTATGCCGTC10.5780346820809248No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA10.5780346820809248No Hit
TTATGAAAGAAGATGCATGGAATTGCGGAGAATCTGTCTCTTATACACATC10.5780346820809248No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA10.5780346820809248No Hit
AAGGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA10.5780346820809248No Hit
ATAGGATGAAGACTAGACTTGAAATTCTAAATTCCAAGGATGAATTCAATT10.5780346820809248No Hit
ACATACGCCCACAATGGTAGCAGCATCGAGCCTTAAAACCATTACCTAATG10.5780346820809248No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAGTCCCGA10.5780346820809248RNA PCR Primer, Index 27 (96% over 28bp)
GTTTATACTGTGGGATGCCAAGTGTAACAGTACCAGGTTTGTGAGGTACTG10.5780346820809248No Hit
GAGTACGGGCTTTTTCGGATTTGGCGACGTGTGGAAAGTAAAAGGATATTT10.5780346820809248No Hit
GTGTATTTCCGATGTTTCGTTTTTCCAACGTTTTCGTCGAAAAATACACTA10.5780346820809248No Hit
GTATCAACGCAGAGTACGGGTAGAGAGAATCGTAAAGACTGTCTCTTATAC10.5780346820809248No Hit
GCCTCTAACCCAGAGGTAATGGGTTCAAGGCTCGTCGCTGCTACCATTGCT10.5780346820809248No Hit
GAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGCTGTCTCTTATACA10.5780346820809248No Hit
ATGTAGAATGTGTCTTGGATTGGCACCATGCTAACTCTGTCTCTTATACAC10.5780346820809248No Hit
GATAGATCAGTGACATGAACGAATGTATCGTTGAATGACGCATATATATCT10.5780346820809248No Hit
TATCAACGCAGAGTACGGGACGAAAGTAGGGCTTAGTGATCCGGTGGTTCT10.5780346820809248No Hit
GTAGAAGATCTTGATGAAGAAGGTGAGGAAGCAAAGGCTGTCTCTTATACA10.5780346820809248No Hit
TATTGTAAGCAATTCTGAATGTATTAGACAACCTGTTTTGCTGTAACAGGG10.5780346820809248No Hit
GTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGAGCC10.5780346820809248No Hit
TGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA10.5780346820809248RNA PCR Primer, Index 31 (96% over 28bp)
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC10.5780346820809248No Hit
TATCAACGCAGAGTACGGGACGCAGAGTATTTTTCTGTCTCTTATACACAT10.5780346820809248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGATACCCCCCCC10.5780346820809248No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACA10.5780346820809248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGCCTTGTCCCCCCCCCGCACCC10.5780346820809248No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT10.5780346820809248No Hit
CTGCAACTGAAAAATGTCATAACAATCTCCTTCTTTGAGTGACTACTAAGC10.5780346820809248No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAG10.5780346820809248No Hit
GGACTCCAGGTATTAACCAGAGCTTTTTCGTTCTGTACAAAAACTGTCTCT10.5780346820809248No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA10.5780346820809248No Hit
GCACANAAGCAAGATATAAGTTGGTGGATTTTGGAGCTGTCTCTTATACAC10.5780346820809248No Hit
GAGTAAAAGTAGTAATTAGAACGTTGCTGTAACTATGGTCTCCCATATTCG10.5780346820809248No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers