FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_44.351000000424d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_44.351000000424d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256599
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT72642.830876192035043No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC60412.354257031399187No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG23670.922450983830802No Hit
AAAAAGGCTTTGGAAAATATCAACTCCCGTCTTCGTCTTGTTATGAAGAGT3910.15237783467589508No Hit
AAGGAATGTGACAATAACGCAAGAAACCTGTCTCTTATACACATCTCCGAG3180.12392877602796581No Hit
GTTTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAAACCATAC2780.10834025074142922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGA207.019284E-445.00487528
GGCTAGT253.879825E-545.0048756
GCCGTCT5550.044.59073316
CCGTCTT5750.042.2572117
TGCTTGA5850.040.38899225
CTGCTTG5750.040.30871224
GCTTGAA5750.040.30871226
CTTCTGC5950.040.08056321
TCTGCTT5900.039.6575823
CGTCTTC6100.039.46379518
ACGGGAC301.13672635E-437.504063
CTTGAAA6450.036.63187427
TTCTGCT6650.035.1849222
TGAAAAA7150.033.67497629
ACTTCTC352.8125246E-432.1463433
GCTAGTT352.8157444E-432.140077
GTCTTCT7500.032.0972219
TTGAAAA7550.031.89087128
TTGGCTA300.00513717330.0032484
CCGATCT300.005142072729.997414