Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pb_44.351000000424d8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 256599 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT | 7264 | 2.830876192035043 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC | 6041 | 2.354257031399187 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG | 2367 | 0.922450983830802 | No Hit |
AAAAAGGCTTTGGAAAATATCAACTCCCGTCTTCGTCTTGTTATGAAGAGT | 391 | 0.15237783467589508 | No Hit |
AAGGAATGTGACAATAACGCAAGAAACCTGTCTCTTATACACATCTCCGAG | 318 | 0.12392877602796581 | No Hit |
GTTTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAAACCATAC | 278 | 0.10834025074142922 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCGGA | 20 | 7.019284E-4 | 45.004875 | 28 |
GGCTAGT | 25 | 3.879825E-5 | 45.004875 | 6 |
GCCGTCT | 555 | 0.0 | 44.590733 | 16 |
CCGTCTT | 575 | 0.0 | 42.25721 | 17 |
TGCTTGA | 585 | 0.0 | 40.388992 | 25 |
CTGCTTG | 575 | 0.0 | 40.308712 | 24 |
GCTTGAA | 575 | 0.0 | 40.308712 | 26 |
CTTCTGC | 595 | 0.0 | 40.080563 | 21 |
TCTGCTT | 590 | 0.0 | 39.65758 | 23 |
CGTCTTC | 610 | 0.0 | 39.463795 | 18 |
ACGGGAC | 30 | 1.13672635E-4 | 37.50406 | 3 |
CTTGAAA | 645 | 0.0 | 36.631874 | 27 |
TTCTGCT | 665 | 0.0 | 35.18492 | 22 |
TGAAAAA | 715 | 0.0 | 33.674976 | 29 |
ACTTCTC | 35 | 2.8125246E-4 | 32.14634 | 33 |
GCTAGTT | 35 | 2.8157444E-4 | 32.14007 | 7 |
GTCTTCT | 750 | 0.0 | 32.09722 | 19 |
TTGAAAA | 755 | 0.0 | 31.890871 | 28 |
TTGGCTA | 30 | 0.005137173 | 30.003248 | 4 |
CCGATCT | 30 | 0.0051420727 | 29.9974 | 14 |