FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_43.351000000424a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_43.351000000424a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138680
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC22901.6512835304297664No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT6150.44346697432939136No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2250.16224401499855784No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG2160.15575425439861554No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1760.12691087395442746No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1580.11393135275454282No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCC207.012097E-444.9996410
GCCGTCT1850.043.78343216
CCGTCTT2000.040.49967217
CGTAATG301.1348055E-437.499738
ATAACGC301.1348055E-437.49973
TCTGCTT2100.037.499723
CGTCTTC2250.036.99970618
CTGCTTG2150.036.62761324
GCATAAT250.002097102636.0126951
GGAATTC250.002100820735.9997130
TAGCCAT250.002100820735.9997124
GTTACGA250.002100820735.9997134
ATATCTA250.002100820735.9997128
GTCCAAC250.002100820735.9997113
GCTTGAA2150.035.5811126
TTCTGCT2350.033.51037222
CTTCTGC2450.033.0609621
CTTGAAA2350.032.55293327
CGTCCAA352.8078177E-432.142612
TAACGCA501.9651761E-631.4997464
Produced by FastQC (version 0.11.2)