FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_42.35100000042488.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_42.35100000042488.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304471
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC88482.90602389061684No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT22340.7337316197601742No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT15290.502182473864506No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT14350.4713092544117502No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA14140.4644120458106027No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC13650.4483185590745916No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA11040.36259610931747194No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA10980.36062547828857267Illumina PCR Primer Index 8 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG10850.35635577772595745No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT10360.3402622909899465No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC9780.32121285771058655No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC8880.291653392277097No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT8710.28606993769521566No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT8690.28541306068558253No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT8610.28278555264705013No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC8350.2742461515218198No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA8330.27358927451218673No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT8170.2683342584351219No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT7840.25749578777617577No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT7840.25749578777617577No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA7820.25683891076654264No Hit
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACA7280.23910323150644888No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC7080.23253446141011788No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT6720.22071067523672205No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA6700.22005379822708895No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA6340.20823001205369313No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC6330.2079015735488766No Hit
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCTGTCTCTTATACAC6160.20231811896699523No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA5960.19574934887066417No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5930.19476403335621453No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA5590.18359712419245183No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC5470.1796558621346532No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT5270.1730870920383222No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA5240.17210177652387254No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA5230.171773338019056No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG5090.1671751989516243No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT5070.1665183219419912No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACCTGTCTCT5040.16553300642754154No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5010.16454769091309188No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4980.16356237539864224No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4970.1632339368938257No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG4930.16192018287455948No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATCTGTCTCTTAT4910.16126330586492638No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCT4860.15962111334084364No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4850.15929267483602708No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA4710.15469453576859538No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTA4700.1543660972637788No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC4590.15075327371079675No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAA4340.14254231109038298Illumina PCR Primer Index 8 (96% over 27bp)
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACTGTCTCTTATACACA4290.14090011856630025No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4270.14024324155666715No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTCC4260.13991480305185058No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTATAC4240.13925792604221748No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4140.13597354099405198No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT4090.13433134846996925No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACACA4080.13400290996515268No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC4080.13400290996515268No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTA4080.13400290996515268No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC4060.13334603295551958No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACT3940.12940477089772096No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA3890.12776257837363822No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC3860.12677726285918858No Hit
TTTTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3810.1251350703351058No Hit
AAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT3750.12316443930620649No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3740.12283600080138996No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG3740.12283600080138996No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3660.12020849276285756No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATC3520.11561035369542583No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC3470.11396816117134308No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATACA3460.11363972266652653No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACTGTCTCTT3440.11298284565689344No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTC3370.11068377612317758No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG3350.11002689911354448No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCT3270.10739939107501208No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCCTGTCTCTTATACACATC3260.10707095257019553No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCCTGTCTCTT3230.10608563705574586No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCACTGTCTCTTATACACATC3210.10542876004611278No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3180.10444344453166311No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACCTGTCTCTTATACACATCTC3170.10411500602684656No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3160.10378656752203No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTA3130.10280125200758036No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG3130.10280125200758036No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA3110.10214437499794725No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACA3090.10148749798831416No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTCCG3080.10115905948349761No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTCT207.021949E-445.0041119
GCCGTCC207.0276344E-444.9967216
GCTTGAA22050.044.80000726
CTTGAAA22150.044.6993427
CTTCTGC23050.044.51599521
GTCTTCT22950.044.51386619
CGTCTTC23050.044.41837318
CCGTCTT23050.044.41837317
TGAAAAA23200.044.32516529
TTGAAAA22400.044.3009228
GCCGTCT23200.044.12393616
TCTTCTG23150.043.934920
TTCTGCT22700.043.61631422
TCTGCTT23350.042.78763223
CCTGAAA850.042.35680827
GAAAAAA24400.042.2374630
GCCTGAA800.042.1913526
TGCCTGA800.042.1913525
TTCTGCC900.040.00364722
AAACTCG451.9215804E-839.9970786
Produced by FastQC (version 0.11.2)