FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_40.35100000042452.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_40.35100000042452.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121301
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC2949224.313072439633636No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG106628.789704948846259No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT94247.769103305001608No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGC4090.33717776440425057No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTC1570.12943009538256076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATGA302.1449468E-645.01360726
TCCCCTA206.9978903E-445.01360732
AGCTATG302.1449468E-645.01360725
CCATAAC207.012129E-444.99505613
TGCCGTC30300.044.47530745
TATTTAC301.13464695E-437.495883
ACGTGTT301.13464695E-437.495888
CGTGTTC301.13464695E-437.495889
TACGTGT301.13464695E-437.495887
TTATGGA250.002100835635.99604415
TTGTCTG250.002100835635.99604422
AATACCC250.002100835635.99604443
ATGCCGT39350.034.18937744
TTACGTG352.8074352E-432.1393246
GCCGTCT352.8074352E-432.13932445
CAGCTAT453.4582783E-530.0090724
AGACTGC300.00513209629.9967026
CCAATCC300.00513209629.99670219
TCGAAGC300.00513209629.99670213
CTTACAG300.00513209629.9967023