FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_4.35100000041fd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_4.35100000041fd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426620
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC99382.329473536168018No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA21010.49247573953401147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.3816042379635272No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA14480.33941212320097514No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11830.27729595424499554No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11460.2686231306549154No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT10600.24846467582391824No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9900.23205663119403686No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9470.22197740377853828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.20228775022268058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.20135014767240167No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA7710.18072289156626506No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7330.17181566733861514No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5300.12423233791195912No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5250.12306033472411045No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.11227790539590267No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4710.11040270029534481No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4600.10782429328207774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10100.043.22007445
ATGCCGT11100.039.32637444
TATGCCG11550.037.9845443
TATCTCG11550.037.59495536
GCGTTAT11700.037.30963532
CCCACGA11800.036.98911719
ACGAGAC11900.036.86734822
TCGTATG11850.036.6431840
GTTATCT12000.036.5601234
CTCGTAT11900.036.4892239
CCACGAG12050.036.40841720
ATGCGTT12000.036.37689630
CAATGCG11950.036.34080528
ATCTCGT11950.036.33654837
CGAGACA11950.036.33654823
CACGAGA12100.036.2579721
TGCGTTA12050.036.22595231
AATGCGT12000.036.18938429
CGTTATC12100.036.0762633
TCTCGTA12100.035.88609338