FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_39.35100000042438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_39.35100000042438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167967
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC7576645.10766995898004No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT1838110.94322098983729No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG111406.632255145355933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGTG207.0185907E-444.9967277
TGCCGTC79950.044.60275745
ACTACTT356.2195923E-638.56862330
GCCGTCT659.094947E-1238.07415445
TCTAGTA250.002102751835.9973838
CCACTAC250.002102751835.9973828
TATCTAG250.002102751835.9973836
CTAGTAT250.002102751835.9973839
CACTACT250.002102751835.9973829
ACCACTA250.002102751835.9973827
ATGCCGT99750.035.7492844
TACACCA352.8064896E-432.150095
GACCACT352.8114006E-432.1405226
AGACCAC352.8114006E-432.1405225
TATGCCG111650.031.9390143
CTCGTAT111000.031.90308639
GTATGCC111700.031.8844342
TCTCGTA110850.031.86506838
TCGTATG111100.031.85411840
CGTATGC111450.031.8348341
Produced by FastQC (version 0.11.2)