FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_38.35100000042401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_38.35100000042401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161081
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC10285763.85420999372986No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT1836111.398613120107274No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG80094.972032704043308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA207.0166425E-444.9981438
CACATCC207.0166425E-444.998147
TTATCTA207.0166425E-444.9981435
TACGGGG207.0166425E-444.9981415
TCAACTT207.0166425E-444.9981430
AGTATGC207.0166425E-444.9981441
CACCATC302.1544638E-644.998147
ACGGGGT207.0166425E-444.9981416
CCTCAAC207.0166425E-444.9981428
CTAGTAT207.0166425E-444.9981439
ACCTCGA207.0166425E-444.9981427
AGACTTC207.0166425E-444.9981425
AGACCCC207.0166425E-444.9981425
GACCTCG207.0166425E-444.9981426
TGCCGTC108300.044.60341645
GAGACCA356.21666E-638.5698324
ATGCCGT128150.037.65938644
CAACTTA301.1358453E-437.49844731
AACTTAT301.1358453E-437.49844732
CGAGACT301.1358453E-437.49844723
Produced by FastQC (version 0.11.2)