FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_37.351000000423f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_37.351000000423f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC107.518796992481203No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT32.2556390977443606No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC32.2556390977443606No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCAACTTATCTCGTATGCCGTC21.5037593984962405No Hit
ATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTCTTCTGCTTG21.5037593984962405TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTCTTCTGCTTGA21.5037593984962405TruSeq Adapter, Index 10 (96% over 28bp)
CTCCTCCTGTTCCTGACAACATCTTGGCTCAAGCCTTTCAGACTTCTCCAG10.7518796992481203No Hit
GAGCAAAGGTCACAACCATACCAGGCTTGATCATACCAGTCTCAACACGTC10.7518796992481203No Hit
TTCATGAACTTAGTTATTGACGATGGTATAGGTAAAAGCTGTCTCTTATAC10.7518796992481203No Hit
GAAGATGGAGAACAAGGTACCGAAGAAGACAAGAGTTTTGTAACACTCTCG10.7518796992481203No Hit
CTGGATTGGAGCAGTTGTCGTTAAGTGTCTTGCCCAAGGACACATACTGTC10.7518796992481203No Hit
ATTTAATTGGCTTGGCTAAACAATGCCATATATCCCCTGTCTCTTATACAC10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCCACTTATCTCGTATGCCGTC10.7518796992481203No Hit
TCTTTAAAGGGAGCAGCGTTTGGAACTCCTGGATCTTTCTTTGATTTCTTA10.7518796992481203No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAACTGTCTCTT10.7518796992481203No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTGAAAACCCA10.7518796992481203RNA PCR Primer, Index 27 (96% over 28bp)
TCTTATACACATCTACGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT10.7518796992481203No Hit
CGCCCACAATGGTAGCAGCGTCGAGCCTTGAACCCATTCCTCTGGGTTACA10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATACTTATCTCGTATGCCGTC10.7518796992481203No Hit
GAATACGAGTTGAACATGAAAGGTTTGATACACCACCTGTCTCTTATACAC10.7518796992481203No Hit
GAGTACGGGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGG10.7518796992481203No Hit
CTTATACACATCTCCGACCACGAGACTCTGGACCATCTCGTATGCCGTCTT10.7518796992481203TruSeq Adapter, Index 7 (95% over 21bp)
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCCTTATCTCGTATGCCGTC10.7518796992481203No Hit
GTAAACAAGAGGGAAGAAGAAGAAGAGAAAGTTCCTAAACTGTCTCTTATA10.7518796992481203No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGTACACCGTC10.7518796992481203Illumina PCR Primer Index 5 (96% over 28bp)
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCCTTTGCTACTGCGTAC10.7518796992481203No Hit
GGGGAAACCCAGTGTGTTTCGACACACTATCATTAACCTGTCTCTTATACA10.7518796992481203No Hit
GATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATGCGGAA10.7518796992481203No Hit
GTATCAACGCAGAGTACGGGATCCTATCACCCCTTAAAATCAGTCTCTTAT10.7518796992481203No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTAACTCGTATGCCGTC10.7518796992481203No Hit
AGATATCTCCGAAGTAAGATCTTTTGATCAAAACAAAACTGAAGAAAGTCG10.7518796992481203No Hit
TTCTTGTCTTCTGATGTCGCTTTAACTTTGGAGAAACTTCTGATGATACAT10.7518796992481203No Hit
CTATAGCATGCAGAAATATTTACATCTCAGACCATCACTCTAAGCAAATAC10.7518796992481203No Hit
TTTGAAAGGAGTAGATCCCAAGTATCTGAAGAACATGAAATTTGCTAAGAA10.7518796992481203No Hit
CTAAATCTCGTCAACTTTGTCATTCATCCATCGAACAATGTATCACCTGTC10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC10.7518796992481203RNA PCR Primer, Index 13 (95% over 21bp)
TATCAACGCAGAGTACGGGTGGTATCAACTGTCTCTTATACACATCTCCGA10.7518796992481203No Hit
GTGCATCATCGTAGTATCTAGTGGTCACATAATGTGCAGCTGTCTCTTATA10.7518796992481203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTCCT10.7518796992481203No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT10.7518796992481203No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCCCCGTACTCCTGTCTCTTATAC10.7518796992481203No Hit
CTTCTCGGTCACGGCCGTTCCGATGGTATCCGCTGCTACATGGGTGATATG10.7518796992481203No Hit
CTCCTCTGTTTCTCCCTTTAGCATCGTTGTTTGGTTTGCAATACCAACTTT10.7518796992481203No Hit
GGTAACATGCTTCTTCCGTTTGCACATGGACATAAGCTCCTTTGGCCGAGC10.7518796992481203No Hit
AGCTAATGCCTAGAGAAGCTGATCCTTGTGCTCCAATCTCTGCTTATAGAT10.7518796992481203No Hit
TGATATTCAAAGATTGCCCAATATTTCATTCTATCACTTTCTCTTATACAC10.7518796992481203No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAAAAAAGG10.7518796992481203RNA PCR Primer, Index 27 (96% over 28bp)
ATATAAAAGAAGGTGAAGGAAATGAAGTACCAAGCAATCAGTCCCTTATAC10.7518796992481203No Hit
GCGGTGTGTTCGTCGAGACCGAGGAAGAGAGGAGTACGAGCTGTCTCTTAT10.7518796992481203No Hit
GGTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.7518796992481203No Hit
TTATTACATTAGCAAAAACGAAAAAAATCAAAACCCAAACCACTATCTTAT10.7518796992481203No Hit
TATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGCTGTCTCTTATAC10.7518796992481203No Hit
ATCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTCTGTCT10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTATTTATCTCGTATGCCGTC10.7518796992481203No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG10.7518796992481203No Hit
ATTCTCAACATGTGGTAAAGATGGAAACGTTTTTGTTTGGGACAACAAGTC10.7518796992481203No Hit
AAGCTCAAGTATCTTGATTTTCTTCGCAAGAGGATAAGAACCAACCTGTCT10.7518796992481203No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAAAAG10.7518796992481203Illumina PCR Primer Index 5 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCCCACCGTATCTCGTATGCCGTC10.7518796992481203No Hit
CTTATACACATCTCCGAGCACGAGACCAGCAGCAATCTCGTATGCCGTCTT10.7518796992481203RNA PCR Primer, Index 36 (95% over 22bp)
GGGAATAAGCATGAATGTGCTTGCTATCGTGACAAGCTCAGTAGCAAAGGC10.7518796992481203No Hit
CTCTTATAACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT10.7518796992481203No Hit
ATATAGCGGTAGCTTAAGAGAACACAGCAAGTTTTTAGCAACAAACCTGTC10.7518796992481203No Hit
ACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTAC10.7518796992481203No Hit
GCGTTATACCACTGTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACA10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCGACTTATCTCGTATGCAGTA10.7518796992481203No Hit
AATATGAGCTAAGACGATGATATGGGCATTCATTCTTCCTCTACGATCTTG10.7518796992481203No Hit
GGGTACAATGTTTAACTAAAGACAGATGCATGATCCGAACTATAACTTTCA10.7518796992481203No Hit
CTACTATAGTGTTTCTGCTGCTATTCATTTAAGATTGGTGAAGAACAGATC10.7518796992481203No Hit
AAACAGAACAAGAATCTTCATCATCACCGTCTTCTTCTTCTTCATGTGGAC10.7518796992481203No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACCTGTCTCTTATACACATCTC10.7518796992481203No Hit
GTATCAACGCAGAGTACGGGAAGCTGTCTCTTATACACATCTCCGAGCCCA10.7518796992481203No Hit
GGTCTAGGAACATTGTTGTTGAATCCAAATCTTCCTTTCTTCTCAGATGCC10.7518796992481203No Hit
GAGATGTGAGGCTTTTTTAATTGATTTAGGCCACCAGAAACGTTACACCAA10.7518796992481203No Hit
GTTCTTACCGGTTTTGAATCTCTCCTCAAGCTTAGCCTTAGCTTCCTTAAG10.7518796992481203No Hit
ATTATATTTGGTGTCATATTGAATGAAAACGTTTGTCTAAAAAAAGATCTT10.7518796992481203No Hit
GTATCAACGCAGAGTACGGGGGCTGTCTCTTATACACATCTCCGAGCCCAC10.7518796992481203No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAACTGTCCCTTATACA10.7518796992481203No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAAAAA10.7518796992481203Illumina PCR Primer Index 5 (96% over 28bp)
GCTCCGACAAGGTAATCAAGTTCCTTCTGCATGAGGAAACCAGCAACTGAA10.7518796992481203No Hit
AGCGTCGGTAAGGTGATATGAACCGTTATACTGTCTCTTATACACATCTCC10.7518796992481203No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGAAAGAATGCA10.7518796992481203RNA PCR Primer, Index 36 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCCCTACTTATCTCGTATGCCGTC10.7518796992481203No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG10.7518796992481203No Hit
CACTATCATTAACTGAATCCATAGGTTAATGCTGTCTCTTATACACATCTC10.7518796992481203No Hit
CCCTTCTGCATATAAGAGTGGCAAATGAATACCTCGCCTTTAAAATTGTTT10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCAACGTATCTCGTATGCCGTC10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCCACTTACCCGTAATGCCTCC10.7518796992481203No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC10.7518796992481203No Hit
ATCTAGCACAACAAGTATTAATAACTCGCTTTACAAATACAGAATTATTTC10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATACTAATCTCGTATGCCGTC10.7518796992481203No Hit
TAACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTCTTCTG10.7518796992481203TruSeq Adapter, Index 10 (95% over 24bp)
GCTTTATCTAAAGACAGAAGGACGATTGTAATGTACCATCCTGAAGAAATG10.7518796992481203No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA10.7518796992481203No Hit
GTTAAAGTAACGAATGTTCACCGGTGTAAGATCTGTCTCTTATACACATCT10.7518796992481203No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG10.7518796992481203No Hit
ACACTACCCAGATAATTCGTTTGATGTCATTTACAGCCTGTCTCTTATACA10.7518796992481203No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCACTTATCTCGTATGCCGTC10.7518796992481203No Hit
CTACACTGATGTATTCAACGAGTTCACACCTTGGCCGCTGTCTCTTATACA10.7518796992481203No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAGACAT10.7518796992481203RNA PCR Primer, Index 27 (96% over 28bp)
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTA10.7518796992481203No Hit
ACAATACATCTTCTAACAGAAGACGTATCTCAAAAACACCAGGTGGTCGTT10.7518796992481203No Hit
GAATACAGGTTGCAAGTCTTCTCTTTTAAGATACATACGAACACCTAATCC10.7518796992481203No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT10.7518796992481203No Hit
GGGATAACTACTGGAAACGGTAGCTAATACCTGTCTCTTATACACATCTCC10.7518796992481203No Hit
CCAAGGAGGAGCACAATATTAGGCTTGGACATATTGCTCAGTGGCTGTCTC10.7518796992481203No Hit
TTAGGTGGTTGGTAAAGCATAGCTGTTTAAATGGCTGTAGCCCAAGACAGT10.7518796992481203No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10.7518796992481203No Hit
GGCTTATATTGATTGTTGTGATGCCTCCCGTACCTGTCTCTTATACACATC10.7518796992481203No Hit
GGTATCAACGCAGAGTACGGGTGGTGGTAGACACCTGTAATCCCAGCTACT10.7518796992481203No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTGAAACACCCCCCGCACCCC10.7518796992481203No Hit
GCTTTGCTCGGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTCTCGTAT10.7518796992481203Illumina PCR Primer Index 2 (95% over 22bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATAAAA10.7518796992481203RNA PCR Primer, Index 27 (96% over 28bp)
CCATTAGGTAATGGGTTCAAGGCTCGTCGCCGCTACCATTGTGCTGTCTCT10.7518796992481203No Hit
CCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCTGTCTCTTATA10.7518796992481203No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers