FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_36.351000000423d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_36.351000000423d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT42.8169014084507045No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC32.112676056338028No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC10.7042253521126761No Hit
ATCCTGATTTGCCTTGATGTATAGTATAACTCCTGTCTCTTATACACATCT10.7042253521126761No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT10.7042253521126761No Hit
GGTAACGTCAATGAGCAAAGGTATTAAGGTTACTCCCTTCCTCCTGTCTCT10.7042253521126761No Hit
CCTTACGGGTTTACTCACCCGTTGACTCGCACACATGTCAGACTCCTTGGT10.7042253521126761No Hit
GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA10.7042253521126761No Hit
GTATCAAGCAGAGTACGGGACGCAGAGTACTTTATCTGTCTCATATACACA10.7042253521126761No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGC10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGAGTTGATCTCGTATGCCGTC10.7042253521126761RNA PCR Primer, Index 23 (95% over 21bp)
GGAAGCAGTGGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTC10.7042253521126761No Hit
GGCCTCATGCATGAGATCTTCAATGAGCCCACTAACTGTCTCTTATACACA10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC10.7042253521126761No Hit
GGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATA10.7042253521126761No Hit
TGCCTGTACTGTCCAATTGTGCAGCTGAATGAATCATTGTCACGTCTGAAC10.7042253521126761No Hit
CTTTTCAATCTGCCATAACATGTGCCCACACATCTTCACCTTGGAAATGTA10.7042253521126761No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT10.7042253521126761No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACGGAAGAA10.7042253521126761Illumina PCR Primer Index 8 (96% over 27bp)
CACCCATCTTGGTGGGGAAGATTTTGACAACAGAATGGTTAACCACTTTGT10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTAACTCGTATGCCGTC10.7042253521126761No Hit
CATTCCGTGTCATCAAGCGCCAGTTCGGTCATGTGAAGGCTGTCTCTTATA10.7042253521126761No Hit
GCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATAT10.7042253521126761No Hit
GCAATCAGTTTCGGAACCTCAGATTCGATACTGAAGTTAGCAAACTTCTCG10.7042253521126761No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.7042253521126761No Hit
GCGCCTCGTGGTGCGACAGGGTCCGGGCACGATGGGGCCTGTCTCTTATAC10.7042253521126761No Hit
GCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATCTGGCTCTTAT10.7042253521126761No Hit
TTTTTTTTTTTTTTTTTTTTGCGTTTTTAACTCCCCTTCCTGGTTTTTATA10.7042253521126761No Hit
GAATCCATGTGATGCTGTACCTCTCAAAGATTTAGACCTGTCTCTTATACA10.7042253521126761No Hit
CTTTTGTATTAAATCTCTCTTTTATGTGTTTGGTTGCTTTGACGATCCCGT10.7042253521126761No Hit
TTTTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTCTGTCTCTTATAC10.7042253521126761No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTC10.7042253521126761No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAACAACAA10.7042253521126761Illumina PCR Primer Index 5 (96% over 28bp)
GTATCAACGCAGAGTACGGGGGGAAACCCAGTGTGTTTCGACACACTATCA10.7042253521126761No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAACCACCCCCTGTCTC10.7042253521126761No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA10.7042253521126761No Hit
GGACTGGGATGATGAAGAAGATGGTGAGTGGACTGCCCCAACAATCCCCAA10.7042253521126761No Hit
AGTCATATTAGGGTGGAGGGGAATTTTTGGACGAATTGTTTTTCACATGAA10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATACATATCTCGTATGCCGTC10.7042253521126761No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10.7042253521126761No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGTAGCCCCAATCGGGCGGTAAATTC10.7042253521126761No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC10.7042253521126761No Hit
TCTTATACACATCTCCGAGCCCACGAGCTGATTAAATCTCGTATGCCGTCT10.7042253521126761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT10.7042253521126761No Hit
TTCTTAGTGTGGAGGTATACCAAACGACCACCTGGTGTTTTTGAGATACGT10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTTCAAATCTCGTATGCCGTC10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC10.7042253521126761RNA PCR Primer, Index 13 (95% over 21bp)
AATCACAACTTTAGCACCTTTGACAGATGGATATCTACCAGCAAAAGTCTC10.7042253521126761No Hit
CTTATACACATCCCCGAGCCCACGAGACGTCTTAGTATCTCGTATTCCGTC10.7042253521126761No Hit
ATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGA10.7042253521126761No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTAATGAACTT10.7042253521126761RNA PCR Primer, Index 27 (96% over 28bp)
GGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAG10.7042253521126761No Hit
GGTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.7042253521126761No Hit
CTTTTATGTTTCTCCTTTCGCTATTCACTTCATGAAGATGGGAATCAACAG10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTAATTATCTCGTATGCCGTC10.7042253521126761No Hit
AAAAGAGTACTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTC10.7042253521126761No Hit
CTTTTTAACTGTTTACAATGTACTCATCCAAACAACAACCAAGATTTGAGA10.7042253521126761No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGATAC10.7042253521126761No Hit
AACTTCTTCCATGGACTTTCCAACAACGACATACGAGTTTATGACGTCCTG10.7042253521126761No Hit
GTCAATGACTTCGAGATGAATTGGCTTAAGTCTGTCTCTTATACACCTCTC10.7042253521126761No Hit
CACAACAATGCTGTTATGGAGCTGTTAAGAGGTATCAGAACTGTCTCTTAT10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCCGTATCTCGTATGCCGTC10.7042253521126761No Hit
GTGCTGTTTGATCAAGCTACTTATGACAAGCTTCTCACTGAAGCCCCCAAG10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCCCGTATGTCGTC10.7042253521126761No Hit
CATCTTCAGAGTTATTACCATCTTTCTTCTTATCCTTCTTCTTCTCCTTCT10.7042253521126761No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10.7042253521126761No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACATGTGGCTGTCTCTTATACA10.7042253521126761No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCTTATCTCGTATGCCGTC10.7042253521126761RNA PCR Primer, Index 27 (95% over 21bp)
CTTCTGCAGAGACTTCAGTTAACGCTGTGTCAACAAATGATGAGTCGCCCC10.7042253521126761No Hit
GTATAATATTTGGATCACTAGCAGTCACTTAGGGTGGCATTGTCTTCAACT10.7042253521126761No Hit
TTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCA10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATCTTATCTCGTATGCCGTC10.7042253521126761No Hit
GTACGGGGAGGATGGTGGATAAAAACAAAAACACCTCTATCACTTAAAAAA10.7042253521126761No Hit
AAAAAGAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACAT10.7042253521126761No Hit
TATTCAGACAATGAGGGGCCAAGCACCATCATTCATCCTGATCTGTCTCTT10.7042253521126761No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCCACTTATCTCGTATGCCGT10.7042253521126761No Hit
AAGGAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATCTGTCTCTTAT10.7042253521126761No Hit
ACCATAGGGATACGGGTAGAACCACCAACACTGTCTCTTATACACATCTCC10.7042253521126761No Hit
CTAGCAAAGAAACTGAAGAATATTGCCGAGCTTGTGCTGTCTCTTATACAC10.7042253521126761No Hit
CCCATGAAGAATGCACTATTGGTTTTTAGACATCTCTCTATCACCATGTTC10.7042253521126761No Hit
ACTGTAATTTGAAGGAAATTTGGAAATGTATGAGCATAAATATCCACACAC10.7042253521126761No Hit
CGTCATAAGCCTGTTCCAAGATAACCATTACTGCCACCAGAACCTGTCTCT10.7042253521126761No Hit
ATGTATAACCGCGGTAGCTGGCACGTTAATTAACCTCTGTCTCTTATACAC10.7042253521126761No Hit
GAAAGGGAAATGTTGGTGAAACGACAATATGACAAAATCCTGGAACTGTCC10.7042253521126761No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT10.7042253521126761No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACACAGACA10.7042253521126761Illumina PCR Primer Index 5 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGTTCTTATCTCGTATGCCGTC10.7042253521126761No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT10.7042253521126761No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCGATTTGAAACCCCCAAATC10.7042253521126761No Hit
ACAACTTTGTCCAAGAGTTCAAGAGGAAGAGTAAGAAGGATATCACCGGTA10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGATGTTCATTAATCTCGTATGCCGTC10.7042253521126761No Hit
CGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCCTGTCCCTTATAC10.7042253521126761No Hit
GTATGAGATGGGAAGGCAGCCAGCCAACACCTGTCTCTTATACACATCTCC10.7042253521126761No Hit
GGATCAGTAAGCAGGGGATGGGGTTCAGTCTAAAATTCTGATTTGTAAATT10.7042253521126761No Hit
TCACAGATCCATTTATTCTTGCCATGAAGTAAACGGTACTTATACAAGTGT10.7042253521126761No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATCTCCGAGC10.7042253521126761Illumina PCR Primer Index 5 (96% over 28bp)
ACTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGAGGTCTTAGTATCTCGTATGCCGTC10.7042253521126761No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAACAAACA10.7042253521126761Illumina PCR Primer Index 5 (96% over 28bp)
CGCAAAAAAAAAAAAAAAAAAAAAAAAAGAACTTTCCGTTGATACCACTGC10.7042253521126761No Hit
TATCAACGCAGAGTACGGGTCGAGGGAGGTCCTAAGAAGAAACGTAAAGTT10.7042253521126761No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTCT10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC10.7042253521126761RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.7042253521126761No Hit
CTACCAAATGCCGTTAGCTGCTTGGCAGGAGTCAACGGAGTTGTAGTCTGT10.7042253521126761No Hit
CCACTGAATCTGCGATGAAGAAGATTGAAGACAACAACACTCTTGTTTTCA10.7042253521126761No Hit
GACTCGCACACATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAAT10.7042253521126761No Hit
TATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGT10.7042253521126761No Hit
AGACCAGAAGGGAAACCAGCACCCCCGTACTCTGCGTTGATACCTGTCTCT10.7042253521126761No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACC10.7042253521126761No Hit
ACCAAATATTTTTCGTGTTGCAAGTGTACCATTTGTATCTGTCTCTTATAC10.7042253521126761No Hit
GTACGGGAAGCAGTGGTATCAAGCAGTGGTATCCTGTCTCTTATACACATC10.7042253521126761No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATCTGTCTCTTATACAC10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCACTTATCTCGTATGCCGTC10.7042253521126761No Hit
GTTATGAAGAGTGGTAAATACAACCTTGGATATCGACAAACACTTCGTATG10.7042253521126761No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT10.7042253521126761No Hit
GTATCAACGCAGAGTACGGGACACAGCTCTTGAAAACCATCTTAAAGCAAC10.7042253521126761No Hit
CATCTGGAACTGAGTCTCTCTGCTTTCCTTCTAATGTGTAAGTAACAGACT10.7042253521126761No Hit
ACAAAGTACAATGCCCCCGAATATTATTCCTACAGTGAATTTTCATATTAT10.7042253521126761No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACG10.7042253521126761No Hit
GGACACAGCTCTTCATTCCATTGACTTAGAGGCAACAGGATCTGTCTCTTA10.7042253521126761No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAATCGTAACAAA10.7042253521126761RNA PCR Primer, Index 27 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTG10.7042253521126761No Hit
TGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATGCATCACC10.7042253521126761RNA PCR Primer, Index 31 (96% over 28bp)
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC10.7042253521126761RNA PCR Primer, Index 20 (95% over 21bp)
CACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA10.7042253521126761No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAGT10.7042253521126761RNA PCR Primer, Index 27 (96% over 28bp)
CTCATGCCCGATTTAACGAAGTGATTAGCACAGATCTTCAAAGTTTTAGAT10.7042253521126761No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACTAATCTCGTATGCCGTC10.7042253521126761No Hit
ATTGTCGACTGCTCGCTGCTAACAGCTCAAAGCGATTAGATCAAGAAGACG10.7042253521126761No Hit
GTATCAACGCAGAGTACGGGACGTTATGTTCTGAACAGAATTGTTACACTT10.7042253521126761No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA10.7042253521126761No Hit
GCGAAGGGGGGACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCCCCT10.7042253521126761No Hit
GTCCTAGAAGGTTGCCCTAGACTGCAGAAACTTGAGATAAGGGACAGTCCC10.7042253521126761No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC10.7042253521126761No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers