FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_35.351000000423bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_35.351000000423bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174660
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC92335.286270468338486No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT20281.1611130195809No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG3160.1809229359899233No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2670.15286843009275164No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2640.1511508072827207No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2550.14599793885262796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.145425397915951No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2150.12309630138554906No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.11050040077865567No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1850.10592007328523989No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1830.10477499141188595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10900.043.34862545
ATGCCGT12950.036.48648544
GACCTCT13100.036.2404626
CACGAGA13000.036.17307721
AGACCTC13200.036.13636425
CGTTCAG250.002102229536.00000410
GTTCGCC250.002102229536.00000424
AGATGTT250.002102229536.0000045
CTCGTAT13000.036.039
CCACGAG13000.036.020
CTTATCT13100.035.89694634
CGAGACC13050.035.86207223
ACTTATC13250.035.8301933
GAGACCT13200.035.79545224
ACCTCTA13300.035.6954927
CTCTACT13300.035.6954929
TATGCCG13250.035.66037843
ATCTCGT13100.035.55343637
TATCTCG13100.035.55343636
TCTCGTA13100.035.55343638
Produced by FastQC (version 0.11.2)