FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_32.35100000042353.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_32.35100000042353.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93939
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC1738418.505625991334803RNA PCR Primer, Index 13 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG1034011.007142933180042No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT80418.559810089526183No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGC4400.46838906098638483No Hit
CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTC2370.25229138057675726No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTC1190.12667795058495407No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGT207.0002046E-445.00000416
TGCCGTC18550.044.6361245
TACGGGG356.1872233E-638.5714315
ATCAATA250.002097290236.02
ACTTTGG250.002097290236.032
ATGCCGT26950.030.72356244
AACTTTG300.005123500730.00000231
GCATGTT300.005123500730.0000029
ACGCAGA803.3105607E-928.1250027
GAGTACG803.3105607E-928.12500212
AGAGTAC803.3105607E-928.12500211
CGCAGAG803.3105607E-928.1250028
GTACGGG803.3105607E-928.12500214
AACGCAG856.320988E-926.4705876
GCAGAGT856.320988E-926.4705879
CAGAGTA856.320988E-926.47058710
AGTACGG856.320988E-926.47058713
TATGCCG36950.022.4086643
GTATGCC37100.022.25741442
TCTCGTA37250.022.0469838
Produced by FastQC (version 0.11.2)