FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_31.3510000004232c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_31.3510000004232c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485450
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC12478625.70522195900711RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT222884.5912040374909875No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG126222.600061798331445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC129750.044.61553645
ATGCCGT153150.037.76928744
TCCGGAC250.002102341536.0124473
CCGGACT250.002102341536.0124474
ATCCCGA250.002106598335.9976110
GCCGTCT1000.035.9976145
GGCTCGT504.8717993E-835.9976121
TATGCCG166300.034.7556643
CTCGTAT167050.034.5726839
GTATGCC167150.034.56545642
CGTATGC167500.034.49323341
ATCTCGT167400.034.48695837
TCTCGTA167250.034.47753538
CAAATCT167450.034.4363534
ACCTATT168050.034.43389527
AGACCTA168250.034.4197125
CCTATTC168000.034.4173628
TCGTATG167750.034.41540
CTATTCA167750.034.41529
AAATCTC167300.034.41343335
Produced by FastQC (version 0.11.2)