FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_30.35100000042302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_30.35100000042302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696425
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC191402.7483217862655707RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT81951.176723983199914No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG15070.2163908532864271No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA7750.11128262196216392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC19550.043.50133545
AGTCTCG250.002107133935.99793212
CACCTCG300.005147327729.9982767
ATGCCGT29000.029.40348244
TATGCCG30450.028.07720443
ATCTCGT30250.027.89095937
AGACCTA30550.027.76635725
GTATGCC30750.027.73011642
GACCTAT30450.027.70975726
CTCGTAT30500.027.5885839
CCACGAG30950.027.5509220
TCTCGTA30700.027.48213638
GAGACCT30950.027.40750124
ACGAGAC31350.027.34489322
CGAGACC31200.027.33212323
CCCACGA31300.027.24284619
CGTATGC31350.027.1276341
ACCTATT31050.027.1018427
CCTATTC31100.027.05826828
CACGAGA31550.027.02891321
Produced by FastQC (version 0.11.2)