FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_29.351000000422e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_29.351000000422e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532155
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC7305813.728706861722618RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT191373.596132705696649No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG60151.130309778166136No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC7330.1377418233409439No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA7130.1339835198391446No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA6480.12176903345829691No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC6180.11613157820559798No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6110.11481617197996825No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC5390.10128627937349081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC351.2109922E-744.996629
TCGGAGC207.032472E-444.99661613
TGCCGTC78000.044.74122645
CAGCGGA250.002106870835.9972959
CGGAGCC250.002106870835.99729514
ATGCCGT98100.035.50525344
GTATCGG401.5547905E-533.7633256
CGGGCCA401.5598009E-533.74746310
TATGCCG104050.033.45015343
GTATGCC104950.033.18473442
CTCGTAT104500.033.15540339
ATCTCGT104500.033.11234337
CGTATGC105100.033.09456341
TCTCGTA104500.033.09081338
TCGTATG105300.032.92487740
AATCTCG104900.032.9217436
AAATCTC105100.032.79487235
CAAATCT105900.032.5683734
TCAAATC106250.032.56696333
AGACCTA107050.032.5547725
Produced by FastQC (version 0.11.2)