FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_28.351000000422da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_28.351000000422da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265645
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC3664713.795478928645371RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT55122.074949650849818No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG52481.9755688983417719No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2950.11105046208285493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC37900.043.98671745
ATGCCGT43700.038.0971844
ATTGCAA250.00210320536.00346423
GACAGTC250.002105150135.9966857
TGTCGCC250.002105150135.99668510
GTATGCC48700.034.2319642
CTCGTAT48400.034.21172739
TATGCCG48700.034.18576443
CGTATGC48650.034.17465641
TCTCGTA48400.034.16524538
TCGTATG48500.034.1411940
AATCTCG48350.034.10751336
ATCTCGT48700.033.9547837
AAATCTC48600.033.79318635
ATTCAAA49050.033.62708731
TTCAAAT49000.033.61548232
TATTCAA48950.033.60384830
CAAATCT48950.033.5975234
TCAAATC49200.033.570333
CTATTCA49100.033.54701629
Produced by FastQC (version 0.11.2)