FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_27.351000000422b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_27.351000000422b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465518
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC87651.8828487835056862RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT20730.44531038542011264No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8580.18431081075275285No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5900.12674053420061093No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT5180.11127389273884146No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5060.10869611916187988No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4920.1056887166554247No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG4890.10504427326118432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.10139242736048874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9350.044.5143645
TAGCGAC301.13881455E-437.5003628
CGACTTA301.13881455E-437.5003622
ACCGACT301.139535E-437.4963320
TAGTCGT301.139535E-437.4963319
ATGCCGT11550.036.03543544
CGCATGA250.002105724836.00034323
GCGCATG250.002105724836.00034322
GGCATCG250.002106835435.9964838
ACGAGAC12250.034.53094522
CACGAGA12200.034.48803721
GACCTAT12050.034.35717426
TATGCCG12050.034.3534843
AGACCTA12100.034.21520625
CCACGAG12300.034.20396820
GAGACCT12150.034.074424
ATCTCGT12150.034.0707437
CGAGACC12300.034.02471523
CTCGTAT12250.033.97626539
AATCTCG12300.033.8381536
Produced by FastQC (version 0.11.2)