FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_24.35100000042247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_24.35100000042247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73718
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC35254.781735804009876No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT11521.5627119563742913No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG4930.6687647521636507No Hit
TTGTTTCTCAGTAGTATCAAGCATACATTTTCCTTTCTGTCTCTTATACAC1290.1749911826148295No Hit
AGATAGTAACATCTGTTGGTTGAGTTGTTATTGAAGGAAGTGTATTAACCT930.1261564339781329No Hit
ATCCTTCAAAGAAAATGAATGATGTTGCATTACGTAAGGCAATTGCTTATG850.11530426761442251No Hit
GAAGTATAGTCACAATGTCACTTTAGTTTCAGGTGTACAGCATAGTAGATA840.11394774681895874No Hit
TGTCAAAAAAGTTCAAAGTGATGCAACTTACAAAGAGGGTTACAACAAAGG830.11259122602349494No Hit
TCTTTATCATCAGTTTTGCTTCTGTGAGATTCAATAGTAACTGTCTCTTAT810.10987818443256735No Hit
ACATAGTAATCATCCACCTTAAATGTGATGTTCCCGTTTTTATCTTTTACC780.10580862204617597No Hit
AGTGGTGATAATTGTTTCTTCAGTGATGATAATTCCTAGAGGGATAGTAAC770.10445210125071216No Hit
GTATTTACCCATAAGAAAGGAAACAACGTATTCATACAAAGCTGTCTCTTA760.10309558045524837No Hit
GATTAAAAGAGGTATCATGACAATGCCTAAGTATGTAACTGTCTCTTATAC750.10173905965978458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG253.8552625E-545.033
AGGTACC206.990351E-445.018
AGTCCAA206.990351E-445.019
GTTCTAG253.8552625E-545.031
CGTATAT206.990351E-445.029
GACGTAT206.990351E-445.027
TAACGGA253.8552625E-545.015
TAACGCT206.990351E-445.037
CTAGTGT253.8552625E-545.026
AATGACG206.990351E-445.024
TAACATC351.1913653E-745.04
AGCATAG206.990351E-445.039
AACGCTT206.990351E-445.038
AACGCAA253.8552625E-545.022
TGTTAGC206.990351E-445.013
ACGCTTT206.990351E-445.039
TTAACGC206.990351E-445.036
GTATGTC302.1378728E-644.9999963
TGCCGTC3950.042.72151645
AATAGTC403.399873E-739.3752
Produced by FastQC (version 0.11.2)