FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_23.3510000004222d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_23.3510000004222d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148918
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC2327515.629406787628092No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT86825.830054123745954No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG73824.957090479324192No Hit
TGTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCC2060.13833116211606386No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1860.12490095220188291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTC253.8692873E-545.010085
AGCGTAT253.8769747E-544.99496517
AAGCGTA253.8769747E-544.99496516
GCGTATT253.8769747E-544.99496518
CCTGGAT207.0173683E-444.994968
TGCCGTC24600.043.7146245
TCCGATG356.2021754E-638.5800673
CCGATGT301.1336591E-437.50844
CGATTAT301.1336591E-437.508429
GGGCGAT301.1336591E-437.508426
GCGATTA301.1336591E-437.508428
TGGGGCG301.1359043E-437.49580424
CGAAGCG301.1359043E-437.49580414
GGGGCGA301.1359043E-437.49580425
TAGTAGC250.002098925936.008064
CCCTGTT250.002102390635.9959745
CCGTCTT702.0008883E-1135.35318817
GCCGTCT702.0008883E-1135.35318816
CTTCCGA401.5455551E-533.76891
CTATTAA352.799457E-432.1608621