FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_22.35100000042203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_22.35100000042203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154310
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC29091.8851662238351372No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT25721.6667746743568141No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG5240.33957617782386107No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA1950.12636899747262004No Hit
ATAAGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTA1930.1250729051908496No Hit
ATACGAGATAAGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACAG1780.11535221307757111No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT1640.10627956710517789No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAATG207.014294E-445.023
GCACACC207.014294E-445.08
TGCCGTC3400.042.3529445
ATCCTAC301.1353477E-437.4999961
CCGGGGG250.002101472336.035
TACGAGA250.002101472336.02
CCCCCAC250.002101472336.022
ATACGAG250.002101472336.01
CCTACAC458.6351974E-735.03
GCTACAG501.9669587E-631.535
TGAGGCG453.471498E-530.026
CGGGGGA300.005133619529.99999836
ACCCCCA300.005133619529.99999821
GGGGGAA300.005133619529.99999837
TACACAG602.396082E-729.9999985
ATGCTAT406.1417837E-428.1251
AGGCGAA406.1417837E-428.12528
ATGAGGC507.1189905E-527.025
ATGCCGT5350.026.9158944
GCGAACC450.001221747125.030
Produced by FastQC (version 0.11.2)