FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_21.351000000421e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_21.351000000421e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269890
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC38561.4287302234243582No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT22330.8273741153803402No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG5160.19118900292711846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4300.043.9520445
ACCTCAC301.1378287E-437.49876441
ATCGCCC301.1378287E-437.49876426
CGCCCCA301.1378287E-437.49876428
CCCTGAC250.002102685236.0054862
TCATAGG352.8121812E-432.1477553
TCGCCCC352.8152418E-432.141827
CGGGATC352.8152418E-432.141817
CTAGAAG300.00513653230.0045723
ACACCGG300.00513653230.0045726
CGGATAT300.005141189329.99901226
TCGGTCA300.005141189329.99901215
TACACCG406.1483396E-428.1292865
CATATAA608.128127E-626.2637351
ATGCCGT7400.025.53969844
CTATCCG450.001224358324.99917635
CCTCAAT450.001224358324.99917618
TATGCCG7600.024.5715643
GCCCCAA551.3647224E-424.54464529
TTGCCGT651.5185311E-524.2299730
Produced by FastQC (version 0.11.2)