FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_20.351000000421ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_20.351000000421ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158871
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC8370.5268425326208056No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT4080.2568121305965217No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG2230.1403654537329028No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC207.0149224E-445.03
CGCGTAC207.0149224E-445.010
TATTCTA356.214357E-638.57142625
TCTAACG301.1354985E-437.50000428
TGCCGTC1350.036.66666445
TTCACAC352.8095185E-432.14285737
GGAGCGC300.005134069430.0000026
TCACACA300.005134069430.00000238
GTACTAT300.005134069430.0000021
TCCGTTC300.005134069430.0000028
ACACGAC453.4721365E-530.014
ACACACG453.4721365E-530.012
CTATTCT453.4721365E-530.024
ATCATAC406.142589E-428.1253
GACAAAC507.1202914E-526.99999818
CACACGA507.1202914E-526.99999813
CGCGCGG450.001221905925.010
CACGTGG450.001221905925.031
GACTGTG450.001221905925.07
ACATAGG450.001221905925.025
Produced by FastQC (version 0.11.2)