FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_16.35100000042155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_16.35100000042155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84113
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC65157.745532795168404No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT11711.392174812454674No Hit
TACCAATATTGTTATTGATTTCATCGAACAAAGACCACAGGATTTATTTTC4020.4779285009451571No Hit
ATTTGTGTGTTACATTTTAACACTTGACTCAGATGAATTATGGCCTTTTAC3050.36260744474694756No Hit
AATAGAATCGAAACGAAATGAATCGAAGAGAATCGATTAGAATCGAAATGA2990.3554741835388109No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG2780.33050776931033254No Hit
ATACTATTCTGGAACATTCTTGTTCAACTTTTTCATGTGGTTATTGGATTT2570.30554135508185415No Hit
GCTAAAAAGGATTACCAAATTCTTATCCATGTTCCTAAATCGTTAACAACA2250.2674972953051252No Hit
GTTATACTAACAGCTACAACTACTGGTAATGGAACATGTAGTCCTATTGTT2240.26630841843710246No Hit
TAATAGCAGTGTACCAAATAAGCAGTAATGAACAAACTGTAAAACTAACTG2240.26630841843710246No Hit
ATATAACACCGAGCAATTAATTTGAGAAATGAACTTTGGCAGTTATTTAGA2170.257986280360943No Hit
CTTTTACAGTATGCTTGTCGTAAACTAAAATAGTTGCATAAGCATTCATTA2100.24966414228478356No Hit
GGAAAATGCTATTGATAAAATGTCGTATCAAGAAATCATCGAAATGATTCA2100.24966414228478356No Hit
GACTTAGTACAAAGTAGAAAGTGACAGAACTGAAATGAAAATATGGTGGAT2060.24490863481269246No Hit
GAATTAGCTGTTACTATTGACAAAGAAGGAAATGTAACTATCGTTACAAAA2020.24015312734060132No Hit
TGTCTTCACACTTTATTTCGTTAAGTTGATCTTCAATTTCTGATATCCTTT2020.24015312734060132No Hit
AACATCAATATCGATGATAGGAGGTACATCTTCTTTGGCATATTTTAACGC1990.23658649673653298No Hit
GTGTTAAGAAAACTGATAGTAAAATACGACATTTCAATCTACAATGAAGAG1980.2353976198685102No Hit
GGAATATTAAATAGTTCCTGAATGAGTGCTTATGATTTCTCAGTATAGAAC1880.22350885118828245No Hit
ATGATCAGGATTCATTTGTGATCATGAATGGATTATTGTGTCATATATTAA1830.21756446684816855No Hit
AATCATAGCTAAACAATAAGATTTCAGTTCAGGATCTTTAATGGCATAGTA1800.2139978362441002No Hit
ACTGACTATTGACTACTGACTATTGACTAATGACTATTGACTAATGACCAA1780.21162008250805464No Hit
AGGTATACCTGTCAAGAATTATGATAAAGGATTACCAAACAGAGAATCGGT1780.21162008250805464No Hit
GTTGATTTATAGGGTGATAACTTCAAAGAAGGATTTTAGGAATAGTGGAAC1700.2021090675638724No Hit
TAGTAAGATCGGATTCAATTTCTTTTTCTTTTCCGCCCAGATATTTGAACA1690.2009201906958496No Hit
AAACCATAACTAATACGACACCAATAATTGCGTACATCGCTAATATGTCTC1620.19259805261969018No Hit
ATATGTTAGATCCTAAATCTAGTCACAGAAGAAGGAAAAGAAAATTGAACC1610.1914091757516674No Hit
GCTCTAATCTTTATTATTTCCCTTCTTCTGCTGACTTTAGGCTTTATCTGC1600.19022029888364464No Hit
ATTCTTTTCGATTCTTTTTGATTCGGTTTGATTCTTTTCGATTCAATTCGA1600.19022029888364464No Hit
ATATTACTCAGTGCATTTTAATGAGAGTGACATGTGTATCCCTGAGAAATG1590.18903142201562184No Hit
AAATTATCTTTGGTGATTACGTTTTTGAATGTGTTATCTTCCAGTAATTCA1580.18784254514759907No Hit
TTATTCAAGTACCAATATGCAATACTGATGAGACAAAAAGAAGTGAAGAAT1560.1854647914115535No Hit
GCAATTGTATAGTCAAGGTTTCTATTTCTAAGTGGCTGATTAAACCATAAA1560.1854647914115535No Hit
GAATTAGTAGGACAAATTGTTGAAGGTCCATTTAATGCAATACCACCTTCT1510.1795204070714396No Hit
AATCACTAAGTGATAATTCATTTATCGAAATCACCTTTAGCTCTTCTTTTT1490.17714265333539403No Hit
GATTAGTGATGTTGAACATTTTTTTCATATGTTTTTTGGCCATTTGCGTAT1480.1759537764673713No Hit
GAAGAAAAGAGTATTTTTTCTAATAGCTCGTTTCTACGTATTTCAAGTTCT1460.17357602273132572No Hit
GAGTTTATTTCTCAAAGTTCTAGTTGTGAACAGTAAATGAGAGGTTTTTAT1460.17357602273132572No Hit
TAAAAACACACTTAACTAATGAAGTCATCAGGAAAATGCAAATTAAAACCA1460.17357602273132572No Hit
ATGTACAATTATTTGCTTGATAACGGAATGACACGAAAAGAATACGAGTGG1450.17238714586330295No Hit
ATAGTGTAATACTGTCATAAAGGCAAACATATAGACCAATGGAATAGAATC1430.17000939212725738No Hit
TTTCAAGATTGTTTCAGAAATTCTGGTTATATTGCATGTCCACATTAATCT1400.16644276152318904No Hit
ACATTACTTGGAATAGTCCAAGGAGTAGTAGTCCAAATTACTAGTGATGTA1360.1616872540510979No Hit
AATAAGTACAGCTATTTTTCCATTGGTTAAATTTCCAATCATAATATCCCA1360.1616872540510979No Hit
AATTATTTGAGACCATTCAGGAAAACTTTGATGCAAATTATATTGGCGAAA1340.15930950031505237No Hit
TTTGAAATTAGTATCTGATGGTGCATAATTTTTGCTTTTTAAACCTATATA1330.1581206234470296No Hit
CAATAACCTAATAACTAAATTGGACTACACATTCGAGAAACAAAAAAATGT1330.1581206234470296No Hit
TCTTTCTCTTTGTAAGGCAATTTGATAGCTGGATATGTATAAATACCTGAA1310.15574286971098403No Hit
AATTAAGCCTTTTATTTATTACTGGCATCATGTAGATGGTAATCTAACCTT1300.15455399284296126No Hit
TTTTAATGTTATTCCATTATGTGCTGATTTCCATAACTTCTGTTGAAAAGT1250.14860960850284735No Hit
GCTTATGATAATGGTTTAGAAAATGGCAATTGGATATACAAAAACAACGGT1250.14860960850284735No Hit
GTAATATCTGTCCTTTTTAGATCATTTTCGTCTAATCGTGAAAATATTCTA1220.14504297789877904No Hit
GATTTGTTTCGACTCATTTCTTTTCTATTCGATTCTTTTGATTCGATTCTT1210.14385410103075624No Hit
ATGATAAAGGATCCATTATTTAGGTTGTTAGAGGGAGAGAACTTGAAACAG1190.1414763472947107No Hit
AATCAGTAGTAAGGTTTATAAAAGCAAAAGTAACAATAATTCCATGCGAGG1180.1402874704266879No Hit
CCCAAAATATAGTCTTTTAGAACCTGTGGAGATAAAAAGGAATAAGTCAAA1160.13790971669064234No Hit
TATTAAGGAAGATAACATGGCATTTTTAATTGCCAACAAAATCAAATCAAT1140.1355319629545968No Hit
TTTTGAATCATTGTGTTCATCAATATTTCTACAGTGGTTGAGAAAAGTCAA1130.134343086086574No Hit
ATACATTACTGAGTGATCTCTTTCTATAATCTCTTTGCAGTATTATTCCAC1120.13315420921855123No Hit
AATATGAAGTCCTTGAATATGGATATGGTATGTGTTTTTCAAGCGTATAAT1110.13196533235052846No Hit
GATTACAAAATCGTAGAAAGAAACTAGATCCATATGAAGCTGTTATTGTTG1110.13196533235052846No Hit
TTATGACAGAGGTATACTCAGACAAGTTTATCAAAACACTTCTATGGAACT1090.12958757861448292No Hit
CTTTATATGTAACCATATTTGACAAACAGAACAATGAAAAAGCAACCATTT1090.12958757861448292No Hit
ACAGGATGTAATCTTAACATAGGATTAGACTCTTCCGAAATTAGGATCGAT1090.12958757861448292No Hit
ATACTAATCAAACTAGCATAATACTGGTTGTCTATTGCTTACTATTATGCG1090.12958757861448292No Hit
GTATATTACTACTTTGTCAAAAAACACTCTGAATTCTTCTAAGAATGCTTT1060.12602094801041458No Hit
AAATAATGATTTGCCATACTATTTATCAGATCAATGCTTTAATTTGTTCCC1060.12602094801041458No Hit
AAATTAAATATCTCAACGAATGTGCTGATTAGGGAGTATTAGTTATTAGTT1050.12483207114239178No Hit
GTAATAATCCAACACTTTTATATGGTTATGGAGGATTTAGCTCAAGTCTTA1040.12364319427436901No Hit
ATTTTCTCTATTCTTCTAGTTGGATAATTTTTCTTTCCCTTTTCTAGGAGA1040.12364319427436901No Hit
GAATATTGCCTTTACATCAATTTGAAAACAATGTTTCCTTTGATGTTTGCT1040.12364319427436901No Hit
GATATGATCCGTATCGTTGAAATGATTGATAGCGTATTAATGAATCAAACT1040.12364319427436901No Hit
AAGAAAATGACACTCTAAAATACAACTAGGTCAAACTTTCTAGAAGATAAA1000.11888768680227788No Hit
ATATCATGTATTTGCATAGTTGGGTTTTCAGTAATTGTGATAAAAAGCAAA990.1176988099342551No Hit
ATATTACGCAAGAAGAAAAAAGTGTTATCTATACAGCAACTGTCAAAAACC980.11650993306623232No Hit
AGTTACGACAGCATACAATCAAATGTTGATACACTTATAAAACGCTTGCAA980.11650993306623232No Hit
TTACTGTATAGATCCATTGTTGTTTCTTATCTTGCTTTCTTTGATTGTACT970.11532105619820954No Hit
ATGTAAACAAAGACAGTGTGATTCATAACAGATACCTGGGAAAGTAAATGT970.11532105619820954No Hit
GAGTAAGTATGTACCAATCTGGAATTTCTGTAAGTACTTAGTTAACGAAGA960.11413217933018678No Hit
CAAATAGATGAACTTTAAGTTGATTCAATTCTACATGGACAACCATAAATA960.11413217933018678No Hit
GTTCATATGTACCAAATAATGATCAAAATGCTAACTTATTCATAGGTTGTC960.11413217933018678No Hit
TCTAAATACAGCTCTCATAAAATGCATAAGAGATCCTTGGTAAGTATCGTA950.112943302462164No Hit
GATGTAAACACAAAACAATACATGCTTTCGGGTTTATATAATTTCAGTTGG950.112943302462164No Hit
AAATTGGATGTTTGTTATCAAAATAACCTTGGTGAACTATTCCATCTACAA930.11056554872611844No Hit
TGGAATGGAATTGAAAGGAATCATCAAATGGACTTGAATGAAATCATTGAA930.11056554872611844No Hit
TAGTTCTTCACATACAAGTATCAGGACATACTGATTAAATAACCCCTGAGT890.10581004125402732No Hit
ATATGGAAGCAGAAGCAATTGTTGATTACATCAACCTCAGATTTAATGATC890.10581004125402732No Hit
GTGTATAGGAGTCTATATTAAGTTGATGGTTGCTTAAGTTCAAACAAATTC890.10581004125402732No Hit
GTATTTAACAGCTTTGTTAGTATTACTCTTTAACGATGCGAGATGATAATA880.10462116438600454No Hit
ATACTCTTTTTGCTGTAACAAAAATTTTACCAGCGGATTTTTTTGTGGCTC860.10224341064995898No Hit
TATGTAAATAGATCGCATCTGTAATAGATACCATCGCAACCTTAAATGTAT860.10224341064995898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGAA302.1415071E-645.00000423
CTGTCCT253.8599435E-545.0000048
TTGTGTC302.1415071E-645.00000436
TACCACC351.1940574E-745.00000441
CCAGGCA253.8599435E-545.00000441
TTTCGTC253.8599435E-545.00000426
GAACTGA302.1415071E-645.00000427
CGTGAAA253.8599435E-545.00000437
CGTCTAA253.8599435E-545.00000429
AGTGGCT253.8599435E-545.00000431
TCTAGTC302.1415071E-645.00000418
CACCTTT253.8599435E-545.00000432
ATCGTGA253.8599435E-545.00000435
GGCTTTA253.8599435E-545.00000440
AGTGCTT253.8599435E-545.00000425
TCGTGAA253.8599435E-545.00000436
CTAGTCA302.1415071E-645.00000419
AGGCATG253.8599435E-545.00000443
TTGACTC551.8189894E-1245.00000424
TCGTCTA253.8599435E-545.00000428
Produced by FastQC (version 0.11.2)