Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pb_15.3510000004213b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 216832 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC | 87431 | 40.32200044273908 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT | 53999 | 24.903612012987015 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG | 5220 | 2.4073937426210152 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG | 394 | 0.18170749704840614 | No Hit |
TTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTCT | 241 | 0.11114595631641086 | RNA PCR Primer, Index 13 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTGGACC | 20 | 7.0218544E-4 | 44.998386 | 38 |
TGCCGTC | 9115 | 0.0 | 44.677498 | 45 |
GCCGTCT | 55 | 2.728484E-9 | 36.81686 | 45 |
ATAGTGG | 25 | 0.0021013368 | 36.007015 | 6 |
ACTCGTA | 25 | 0.002103718 | 35.998707 | 38 |
TAGTGGT | 25 | 0.002103718 | 35.998707 | 7 |
TAACTTA | 25 | 0.002103718 | 35.998707 | 9 |
TCTTATA | 7685 | 0.0 | 33.33233 | 1 |
GATTCCA | 30 | 0.0051274737 | 30.012768 | 1 |
TGTACAC | 30 | 0.0051332638 | 30.005846 | 3 |
GCAAAGT | 30 | 0.0051390585 | 29.998926 | 16 |
GTTAGTT | 30 | 0.0051390585 | 29.998926 | 30 |
TGGTTAT | 30 | 0.0051390585 | 29.998926 | 19 |
TTGACTT | 30 | 0.0051390585 | 29.998926 | 32 |
TCAGAGC | 30 | 0.0051390585 | 29.998926 | 13 |
CGAGCCA | 30 | 0.0051390585 | 29.998926 | 15 |
GATTAAA | 30 | 0.0051390585 | 29.998926 | 11 |
CAGAGCC | 30 | 0.0051390585 | 29.998926 | 14 |
ATGCCGG | 45 | 3.4787816E-5 | 29.998924 | 45 |
CTGAAAT | 40 | 6.151177E-4 | 28.123991 | 9 |