FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_15.3510000004213b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_15.3510000004213b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216832
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC8743140.32200044273908No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT5399924.903612012987015No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG52202.4073937426210152No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG3940.18170749704840614No Hit
TTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTCT2410.11114595631641086RNA PCR Primer, Index 13 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGACC207.0218544E-444.99838638
TGCCGTC91150.044.67749845
GCCGTCT552.728484E-936.8168645
ATAGTGG250.002101336836.0070156
ACTCGTA250.00210371835.99870738
TAGTGGT250.00210371835.9987077
TAACTTA250.00210371835.9987079
TCTTATA76850.033.332331
GATTCCA300.005127473730.0127681
TGTACAC300.005133263830.0058463
GCAAAGT300.005139058529.99892616
GTTAGTT300.005139058529.99892630
TGGTTAT300.005139058529.99892619
TTGACTT300.005139058529.99892632
TCAGAGC300.005139058529.99892613
CGAGCCA300.005139058529.99892615
GATTAAA300.005139058529.99892611
CAGAGCC300.005139058529.99892614
ATGCCGG453.4787816E-529.99892445
CTGAAAT406.151177E-428.1239919