FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_14.35100000042111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_14.35100000042111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353910
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC75182.1242688819191318No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT24160.6826594331892288No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4630.13082422084710804No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4590.129693989997457No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3870.10934983470373824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7350.042.54995345
GCCGTCT250.00210540235.99909645
CCGTAAA352.8098733E-432.1556781
GATAAGG352.8168742E-432.1420482
ATGCCGT10300.030.36331644
GGATAAG453.4746132E-530.0119691
GCACAGA300.00513247930.0119691
GAGGGAC300.005136028430.00772533
CTTAGTG300.00514313329.9992473
GCATCCA300.005146688329.99500714
TATGCCG10650.029.36546143
CTCGTAT10450.029.27742439
ATCTCGT10500.029.13800837
CCACGAG10850.029.02742820
GACATAG10700.029.02616526
ACGAGAC10900.028.90244122
GTATGCC10750.028.88299642
TCTCGTA10600.028.86312338
AATCTCG10550.028.78667836
CACGAGA10950.028.77046621
Produced by FastQC (version 0.11.2)