FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_13.351000000420e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_13.351000000420e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448497
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC82211.8330111461169192No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT11280.25150669904146516No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7440.16588739724011534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.1438136709944548No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5970.13311125826928608No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5650.12597631645250693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9250.043.29590645
ATGCCGT10200.039.26344344
ACTCGAT356.2411527E-638.57018717
CTCGATA356.2411527E-638.57018718
CGAGACA10350.038.47702423
TATGCCG10450.038.32412743
CGTATGC10500.038.1416341
GACATAG10300.038.00848426
GTATGCC10650.037.81568542
GCCCACG10550.037.747618
ACGAGAC10650.037.3931622
CCCACGA10750.037.25461219
TCTCGTA10700.037.21842638
TCGTATG10700.037.21842640
CTCGTAT10750.037.0453239
CCACGAG11000.036.61245320
AGCCCAC10950.036.57416517
ATCTCGT11050.036.24317637
AGACATA10900.036.1226925
TGAGTGG504.869071E-835.9988410
Produced by FastQC (version 0.11.2)