FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_12.351000000420dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_12.351000000420dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400350
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC158773.9657799425502684No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT19950.4983139752716373No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA18920.47258648682402893No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA14630.36543024853253403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.3167228674909454No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12100.3022355438990883No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10150.2535281628574997No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9940.24828275259148247No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8310.20756837766953917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.18558761084051456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.1830897964281254No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7230.18059198201573623No Hit
ATATGAAACTGTTGAAAAATGAAAATCAAAATTGAAATTCAGTAAGACTTT6860.17135006868989636No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA6550.16360684401148995No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5890.1471212688897215No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5630.14062695141750967No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG5190.1296365680029974No Hit
ATATAAAGTCAAAGCAATGTGAAGAATACTAGTGGCACAATTGTTATTGGA4970.12414137629574123No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4850.12114399900087425No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4370.10915448982140627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15600.043.98953245
ATGCCGT17800.038.42621644
TATGCCG18100.037.7893243
CTAGGTA301.1387233E-437.4992739
CGTATGC18250.037.47872541
CGAGACA18200.037.4580623
GTATGCC18300.037.37632442
ATCTCGT18400.037.2954737
CCACGAG18400.037.2954720
CTCGTAT18350.037.2744839
TCTCGTA18350.037.2744838
TCGTATG18400.037.1731940
CACGAGA18500.037.09387621
CCCACGA18500.037.09387619
ACGAGAC18600.036.89444422
AATCTCG18500.036.85063636
CATAGAC18600.036.7734828
GCCCACG18800.036.50195318
ATAGACA18700.036.45651229
TAGACAA18750.036.35929530
Produced by FastQC (version 0.11.2)