FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_1.35100000041f66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_1.35100000041f66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324342
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC32711.0085033698996737No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4470.1378174889468524No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT3590.11068563429959735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACAAT3450.038.48261325
CACGAGA3700.036.48498521
TATGCCG3650.036.36836643
GTATGCC3650.036.36836642
GTTATCT3650.036.36836634
CTCGTAT3700.035.876939
TGCCGTC3850.035.64788445
AATGCGT3750.035.40400329
ACAATGC3750.035.40400327
CGAGACA3750.035.39854423
TGCGTTA3900.035.19628531
ACGAGAC3850.035.06349622
CGTATGC3800.034.93277441
GAGACAA3800.034.93277424
TATCTCG3900.034.6139636
TTATCTC3850.034.47910335
TCTCGTA4000.033.7486138
ATGCCGT4050.033.33196344
ATCTCGT4000.033.18613437
CGTTATC4050.032.78148333
Produced by FastQC (version 0.11.2)