FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_96.35100000041f4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_96.35100000041f4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC2968.537640611479667No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT140.4038073262186328No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG130.37496394577444475No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT60.17306028266512835RNA PCR Primer, Index 18 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC206.0995086E-445.00000417
GTATGCC206.0995086E-445.00000442
AATCTCG206.0995086E-445.00000436
ATCTCGT206.0995086E-445.00000437
ATCTCCG206.0995086E-445.00000410
CACATCT206.0995086E-445.0000047
CAGCAGC206.0995086E-445.00000429
CAGCAAT206.0995086E-445.00000432
TATACAC206.0995086E-445.0000043
TGCCGTC206.0995086E-445.00000445
CCAGCAG206.0995086E-445.00000428
CCACGAG206.0995086E-445.00000420
TATGCCG206.0995086E-445.00000443
CATCTCC206.0995086E-445.0000049
CAATCTC206.0995086E-445.00000435
GAGCCCA206.0995086E-445.00000416
CACGAGA206.0995086E-445.00000421
CCCACGA206.0995086E-445.00000419
ACATCTC206.0995086E-445.0000048
TACACAT206.0995086E-445.0000045