FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_92.35100000041ec4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_92.35100000041ec4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11189
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC317428.367146304406116No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT3012.6901421038519975No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG880.7864867280364644No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT170.15193493609795336RNA PCR Primer, Index 18 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCT120.1072481901867906RNA PCR Primer, Index 36 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG206.736701E-445.00000412
GAGTACG206.736701E-445.00000411
GTACGGG206.736701E-445.00000413
TGCCGTC4200.043.92857445
CCACGAG4400.041.9318220
CACGAGA4400.041.9318221
CCCACGA4400.041.9318219
ACGAGAC4400.041.9318222
AGACCAG4400.041.9318225
GAGACCA4400.041.9318224
CAGCAGC4300.041.86046629
CCAGCAG4300.041.86046628
GCAGCAA4300.041.86046631
AGCCCAC4450.041.46067417
GTATGCC4450.041.46067442
GCCCACG4450.041.46067418
TATGCCG4450.041.46067443
GAGCCCA4450.041.46067416
CGTATGC4450.041.46067441
CGAGCCC4450.041.46067415
Produced by FastQC (version 0.11.2)