FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pa_9.35100000041461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pa_9.35100000041461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279028
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC47181.6908697335034477No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT8150.2920853821121895No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5810.208222830683659No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAA207.027542E-444.99551813
TGCCGTC5550.032.83456845
CGTATGC5650.032.25342641
TATGCCG5650.032.25342643
GTATGCC5650.032.25342642
TCGTATG5650.032.25342640
CGAGACA5600.032.14541623
GAGACAT5750.031.3068424
GACATAG5700.031.18669326
CCCACGA5800.031.03695319
ATAGACA5750.030.91550629
AGACATA5750.030.91550625
GCCCACG5900.030.89229218
CTCGTAT5900.030.88675339
ATGCCGT5900.030.88675344
GACAAAT5800.030.6489932
AATCTCG6050.030.49283236
TAGACAA5850.030.38703530
ACGAGAC6000.030.37741922
CATAGAC5900.030.12951928
Produced by FastQC (version 0.11.2)