H23LNBCXX_l01n01_bir_1228_pa_88.35100000041e5b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pa_88.35100000041e5b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34975
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	6173	17.64974982130093	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT	827	2.364546104360257	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG	248	0.7090779127948534	No Hit
ATACTAACTGTAATCTGGTAAACCCTTTGATTTTAAATGCTTTTGCAAAAT	80	0.22873481057898498	No Hit
ACTATAAGGAAGCTACATACGACTATCTATTTAACAGAATCAAACAAATCA	76	0.21729807005003576	No Hit
AGTTAGAATAATCCTAATGACTACTGACTAATGACCATTGACCTTAATTGA	69	0.19728377412437456	No Hit
ATTACTAACAGAAACACTGCATAATCATTTCATAAGTTCGTTTGAAAACCA	58	0.1658327376697641	No Hit
TAGGAGTAACAGCATCTATTATACTTGTGGGTCCTGTAGGAAAGTATTTTG	56	0.16011436740528948	No Hit
TAGCATACTACTCTGCATGAAGATTTTTCAATTCTTCTATCACTTCGCTAT	55	0.15725518227305219	No Hit
TGTGTATTGATGTGTATTGATGGGAATACTCATTCATAGAGGAAGCAGATA	54	0.15439599714081487	No Hit
ATATCAGGTGAATGATCTGCGATTGTATTCTCAAAACGATATTCGTTTTCT	51	0.14581844174410294	No Hit
ACTCATTTGTTTTGTTTCTTAAGTTCCACATTGGAGTGAAATCATATGGTA	51	0.14581844174410294	No Hit
AAATAAGACCTACTATAGTCTCACCCTAATAAAGACTACATTCAAACTACA	50	0.14295925661186562	No Hit
GAAAAGAATCGAATCAAATTTAATGGAATCGAAAAGAATCGAATTGAATCG	48	0.13724088634739098	No Hit
GTATTATAATGAAGCGGGTAAACCATTTGATACAAAATTGATTTACGATGA	46	0.13152251608291637	No Hit
CTATGATATTTACCTCTTTGACACAATCGCTAATGATTTTAAATACCTAAC	42	0.12008577555396713	No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA	42	0.12008577555396713	No Hit
GACAAAGATAAATTCCTAGAAAAGGAAATTGAACGCAATTTAGAGCTCTTC	41	0.11722659042172981	No Hit
GCTTTATAGATTTTCTGAATTTCAGCATCATAGAAAGCCTTATCTTTTCCT	41	0.11722659042172981	No Hit
ATGTATGAAGAAGAAAAAAAAGTGGGCAATGAATTTGAAGTAGATGTTTCC	40	0.11436740528949249	No Hit
CCTTGAATAATTGGTGTATTATCTCCATCGAAACCATAAGAGCTTAATAAC	39	0.11150822015725517	No Hit
ATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCTTC	39	0.11150822015725517	RNA PCR Primer, Index 24 (95% over 22bp)
AAAGTAGACATACAGACTAATGGAACAGAATTGAGAGTCCAGAAATAAATG	38	0.10864903502501788	No Hit
TAGTAGTTCTTGACGTAGCAAAATAGTAAATGGTTTAGCCACAAAATTTAG	38	0.10864903502501788	No Hit
TGTTTATTTAGTGCATTAAAAGCAAGCCGAATAAAAGAGATCACATGAGGG	37	0.10578984989278056	No Hit
TACTTAACCCGATTTAAATTAAATATCCACCAAACAACTTCAATTCGAAAG	36	0.10293066476054324	No Hit
GACTTTAGACTGTAAAACTGAAGTGAGATATGACTTCATATCTATAGGGCT	35	0.10007147962830593	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	655	43.62457	45
ATGCCGT	785	36.40012	44
GAGCCCA	790	36.16974	16
CTCCGAG	785	36.113503	12
AGCCCAC	795	35.942253	17
GTATGCC	795	35.942253	42
CCACGAG	795	35.942253	20
CACGAGA	795	35.942253	21
CCCACGA	795	35.942253	19
ACGAGAC	795	35.942253	22
GACGTCT	795	35.942253	26
TCGTATG	795	35.942253	40
TCTCCGA	795	35.942253	11
ACGTCTT	790	35.884937	27
AGTATCT	790	35.884937	34
TCTCGTA	790	35.884937	38
TATCTCG	790	35.884937	36
TCCGAGC	790	35.884937	13
CTCGTAT	790	35.884937	39
ATCTCCG	800	35.717613	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content